Maggio Anna, Bernardino Raquel L, Gena Patrizia, Alves Marco G, Oliveira Pedro F, Calamita Giuseppe
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari "Aldo Moro", Bari, Italy.
Unit for Multidisciplinary Research in Biomedicine (UMIB), Laboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Methods Mol Biol. 2018;1748:279-286. doi: 10.1007/978-1-4939-7698-0_19.
Movement of water into and out of the cell is fundamental to life. In the male reproductive tract, at the level of the seminiferous epithelium, Sertoli cells are pivotal in mediating the movement of water into the luminal compartment, a process providing a means of transport of spermatozoa into the epididymal ducts. Here, we describe a stopped-flow light-scattering methodology to easily assess the Sertoli cell osmotic water permeability. The devised methodology is well suited for studying how the Sertoli cell's permeability changes following exogenous stimuli.
水进出细胞的运动是生命的基础。在男性生殖道的生精上皮水平，支持细胞在介导水进入管腔隔室的过程中起关键作用，这一过程为精子运输到附睾管提供了一种方式。在此，我们描述了一种停流光散射方法，以轻松评估支持细胞的渗透水通透性。所设计的方法非常适合研究支持细胞通透性在外源刺激后如何变化。
