Multidisciplinary Institute for Health, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.
Department of Biology and Biotechnology of Microorganisms, State University of Santa Cruz, Ilhéus, Bahia, Brazil.
Eur J Nutr. 2019 Apr;58(3):1137-1146. doi: 10.1007/s00394-018-1628-y. Epub 2018 Feb 16.
Lactobacillus plantarum Lp62 is a lactic acid bacteria strain that has been isolated from cocoa beans and exhibited probiotic potential. The influence of oral administration of L. plantarum Lp62 on the growth of rat's pups; on yield, cytokines and milk composition was studied.
Lactobacillus plantarum Lp62 is a lactic acid bacteria strain that has been isolated from cocoa beans. It was administered daily by gavage to Wistar rats (n = 8), from the 7th day before delivery and for 20 days during lactation, in a concentration of 1.44 × 10 CFU/rat. The dam and pups were weighed and milk was collected at 12th and 19th day for determination of protein, triglycerides, cholesterol and lactose by colorimetric assays. TGF-β1 milk levels were analyzed by ELISA. The mammary glands of rats were removed for histological analysis. To detect statistical differences between the groups, tests of mean differences at a significance level of 5% was performed.
Supplementation with L. plantarum L62 resulted in significant higher weight of pups (p < 0.05), with similar weight on dams (p > 0.05). The milk yield was not altered by L. plantarum treatment, but the levels of protein, triglycerides and cholesterol were increased (p < 0.05), with no difference in lactose concentration (p > 0.05). Levels of TGF-β1 were higher in the milk of L. plantarum treatment (p < 0.05).
The treatment of dams at the end of pregnancy and lactation with L. plantarum Lp62 increased nutritional content of milk, probably contributing to the higher weight of the pups. The higher levels of TGF-β1 in the milk, could promote immune benefits to the pups. Further studies in this field are needed to prove the potential use of L. plantarum Lp62 as a probiotic.
植物乳杆菌 Lp62 是从可可豆中分离出来的一种益生菌，具有益生菌潜力。本研究旨在探讨口服植物乳杆菌 Lp62 对大鼠幼仔生长、产奶量、细胞因子和乳成分的影响。
植物乳杆菌 Lp62 是从可可豆中分离出来的一种乳酸菌。从分娩前第 7 天开始，通过灌胃每天给 Wistar 大鼠（n=8）喂食，在哺乳期内连续喂食 20 天，浓度为 1.44×10 CFU/大鼠。称重母鼠和幼仔，在第 12 天和第 19 天收集乳汁，通过比色法测定蛋白质、甘油三酯、胆固醇和乳糖的含量。通过 ELISA 分析 TGF-β1 奶水平。切除大鼠的乳腺进行组织学分析。为了检测组间的统计学差异，采用具有 5%显著性水平的均值差异检验。
补充植物乳杆菌 L62 可显著增加幼仔体重（p<0.05），但对母鼠体重无显著影响（p>0.05）。乳产量不受植物乳杆菌处理的影响，但蛋白质、甘油三酯和胆固醇水平增加（p<0.05），乳糖浓度无差异（p>0.05）。植物乳杆菌处理组的 TGF-β1 水平较高（p<0.05）。
在妊娠末期和哺乳期用植物乳杆菌 Lp62 处理母鼠可增加乳汁的营养成分，可能有助于幼仔体重增加。乳汁中 TGF-β1 水平升高，可能对幼仔有免疫益处。需要进一步研究证明植物乳杆菌 Lp62 作为益生菌的潜在用途。
