Fåk Frida, Ahrné Siv, Molin Göran, Jeppsson Bengt, Weström Björn
Department of Cell and Organism Biology, Lund University, Helgonavägen 3B, Lund SE-223 62, Sweden.
Br J Nutr. 2008 Aug;100(2):332-8. doi: 10.1017/S0007114507883036. Epub 2008 Jan 8.
After birth, the gastrointestinal (GI) tract undergoes vast structural and functional adaptations to be able to digest mother's milk and later, during the weaning period, solid food. Studies on germ-free animals have shown the role of the gut microbiota for stimulating GI maturation, but which groups are involved is unclear. In the present study, we administered the probiotic bacterium, Lactobacillus plantarum 299v (Lp299v), in the drinking water to pregnant and lactating rat dams until their pups had reached an age of 14 d. It was found that Lp299v colonizing the mothers were also able to colonize the pups, which had an impact on their gut growth and function. The small intestine, pancreas and liver weighed more in the 14 d-old pups born from dams exposed to Lp299v than in the control pups from dams given only water. Furthermore, the Lp299v pups showed decreased gut permeability. Despite a heavier spleen in the Lp299v pups, as compared to the control pups, no significant increase in the acute-phase protein, haptoglobin, was found. In conclusion, the results reported here clearly show that manipulating the maternal microflora by exposing expecting mothers to a Gram-positive, probiotic bacterium prior to parturition and during lactation impacts the gut growth and function in the offspring.
出生后，胃肠道会经历巨大的结构和功能适应性变化，以便能够消化母乳，随后在断奶期消化固体食物。对无菌动物的研究表明肠道微生物群在刺激胃肠道成熟方面的作用，但具体涉及哪些菌群尚不清楚。在本研究中，我们在饮用水中给怀孕和哺乳的大鼠母鼠施用益生菌植物乳杆菌299v（Lp299v），直至其幼崽达到14日龄。结果发现，定殖于母鼠的Lp299v也能够定殖于幼崽，这对幼崽的肠道生长和功能产生了影响。与仅饮用清水的母鼠所生的对照幼崽相比，暴露于Lp299v的母鼠所生的14日龄幼崽的小肠、胰腺和肝脏重量更重。此外，Lp299v组幼崽的肠道通透性降低。尽管与对照幼崽相比，Lp299v组幼崽的脾脏更重，但未发现急性期蛋白触珠蛋白有显著增加。总之，本研究报告的结果清楚地表明，在分娩前和哺乳期让怀孕母鼠接触革兰氏阳性益生菌来操纵母体微生物群，会影响后代的肠道生长和功能。
