Hamlyn Centre for Robotic Surgery, Imperial College London, South Kingston Campus, London, United Kingdom.
Institute of Medical Robotics, Shanghai Jiao Tong University, Shanghai, China.
J Biomed Opt. 2019 Nov;24(11):1-7. doi: 10.1117/1.JBO.24.11.116501.
Fiber bundle fluorescence endomicroscopy is an effective method for in vivo imaging of biological tissue samples. Line-scanning confocal laser endomicroscopy realizes confocal imaging at a much higher frame rate compared to the point scanning system, but with reduced optical sectioning. To address this problem, we describe a fiber bundle endomicroscopy system that utilizes the HiLo technique to enhance the optical sectioning while still maintaining high image acquisition rates. Confocal HiLo endomicroscopy is achieved by synchronizing the scanning hybrid-illumination laser line with the rolling shutter of a CMOS camera. An evident improvement of axial sectioning is achieved as compared to the line-scanning confocal endomicroscopy without the HiLo technique. Comparisons are also made with epifluorescence endomicroscopy with and without HiLo. The optical sectioning enhancement is demonstrated on lens tissue as well as porcine kidney tissue.
纤维束荧光内窥技术是一种对生物组织样本进行体内成像的有效方法。与点扫描系统相比，线扫描共聚焦激光内窥技术实现了更高帧率的共聚焦成像，但光学切片减少。为了解决这个问题，我们描述了一种纤维束内窥系统，该系统利用 HiLo 技术在保持高图像采集速度的同时增强光学切片。通过将扫描混合照明激光线与 CMOS 相机的卷帘快门同步，实现共聚焦 HiLo 内窥。与没有 HiLo 技术的线扫描共聚焦内窥相比，轴向切片得到明显改善。还与具有和不具有 HiLo 的荧光外差内窥进行了比较。在镜头组织和猪肾组织上都证明了光学切片增强效果。
