Laboratory of Oxidative Stress, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Konstantynów Ave. 1J, 20-708, Lublin, Poland.
Laboratory of Oxidative Stress, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Konstantynów Ave. 1J, 20-708, Lublin, Poland.
Chem Biol Interact. 2018 Mar 25;284:112-125. doi: 10.1016/j.cbi.2018.02.016. Epub 2018 Feb 14.
The impact of vanadium (V) and magnesium (Mg) applied as sodium metavanadate (SMV, 0.125 mg V/ml) and magnesium sulfate (MS, 0.06 mg Mg/ml) on oxidative stress markers in bone of male Wistar rats was investigated. Some of them were also measured in the liver, e.g. l-ascorbic acid (hepatic L-AA). Additionally, relationships between selected indices determined in bone were examined. SMV alone (Group II) did not significantly alter the level of TBARS and the activity of SOD, compared with the control (Group I), but it slightly reduced the GR activity (by 13%) and the L-AA level (by 15.5%). It also markedly lowered the activity of CAT and GPx (by 34% and 29%), and to some degree elevated the activity of GST (by 16%) and the hepatic L-AA level (by 119%). MS alone (Group III) decreased the TBARS level (by 49%), slightly lowered the L-AA concentration (by 14%), and reduced the SOD, GPx, and GR activities (by 31%, 40%, and 28%), but did not change the activity of CAT, compared with the control. Additionally, it elevated the GST activity (by 56%) and the hepatic L-AA level (by 40%). In turn, the SMV + MS combination (Group IV) reduced the TBARS level (by 38%) and the SOD, CAT, GPx, and GR activities (by 61%, 58%, 72%, and 40%) but elevated the GST activity (by 66%), compared with the control. The activity of SOD and GPx in the rats in Group IV was also reduced, compared with Group II (by 61% and 61%) and Group III (by 44% and 54%). In turn, the activities of CAT and GR were decreased, compared with Group III (by 55%) and Group II (by 31%), and the L-AA level was lowered, in comparison with Groups II and III (by 53% and 54%). Further, the concentration of V in the bone of rats in Groups II and IV increased, whereas the concentration of Mg decreased, compared with Groups I and III, in which the V and Mg levels dropped and were not altered, respectively, compared with Group I. The total content of Fe in the bone of rats in Groups II and IV increased, compared with Group III, in which the total Fe content did not change, compared with Group I. In turn, the total bone Cu content significantly decreased in the rats in Groups III and IV, compared with Groups I and II, whereas the total Zn content and the Ca concentration did not change markedly. The results provided evidence that the concentration of V used as SMV did not enhance LPO in bone, whereas Mg, at the selected level, markedly reduced LPO in this tissue. On the other hand, both elements administered separately and in combination disrupted the antioxidant defense mechanisms and homeostasis of some metals in bone tissue, which consequently may have contributed to disturbances in the balance in the activities of osteoblastic and osteoclastic cells, and thereby negatively affected bone health.
研究了作为偏钒酸钠（SMV，0.125mgV/ml）和硫酸镁（MS，0.06mgMg/ml）的钒（V）和镁（Mg）对雄性 Wistar 大鼠骨中氧化应激标志物的影响。其中一些也在肝脏中进行了测量，例如 L-抗坏血酸（肝脏 L-AA）。此外，还检查了在骨骼中确定的一些选定指数之间的关系。与对照组（I 组）相比，单独的 SMV（II 组）并未显著改变 TBARS 水平和 SOD 活性，但它轻微降低了 GR 活性（降低了 13%）和 L-AA 水平（降低了 15.5%）。它还明显降低了 CAT 和 GPx 的活性（降低了 34%和 29%），并在一定程度上提高了 GST 的活性（提高了 16%）和肝脏 L-AA 水平（提高了 119%）。单独的 MS（III 组）降低了 TBARS 水平（降低了 49%），轻微降低了 L-AA 浓度（降低了 14%），并降低了 SOD、GPx 和 GR 活性（降低了 31%、40%和 28%），但与对照组相比，CAT 活性没有变化。此外，它提高了 GST 活性（提高了 56%）和肝脏 L-AA 水平（提高了 40%）。相反，SMV+MS 联合（IV 组）降低了 TBARS 水平（降低了 38%）和 SOD、CAT、GPx 和 GR 活性（降低了 61%、58%、72%和 40%），但提高了 GST 活性（提高了 66%），与对照组相比。与对照组（I 组）相比，IV 组大鼠的 SOD 和 GPx 活性也降低了（降低了 61%和 61%），与 II 组（降低了 44%和 54%）和 III 组（降低了 44%和 54%）相比，CAT 和 GR 活性也降低了。与 II 组和 III 组相比，L-AA 水平降低（降低了 53%和 54%）。此外，与 I 组相比，II 组和 IV 组大鼠骨中 V 的浓度增加，而 Mg 的浓度降低，I 组和 III 组大鼠骨中 V 和 Mg 的浓度分别下降，没有变化。与 III 组相比，II 组和 IV 组大鼠骨中总铁含量增加，而 III 组大鼠骨中总铁含量没有变化，与 I 组相比。相反，III 组和 IV 组大鼠的总骨铜含量显著降低，而 II 组和 IV 组大鼠的总锌含量和钙浓度没有明显变化。结果表明，作为 SMV 使用的 V 浓度不会增强骨中的 LPO，而在所选水平下的 Mg 会显著降低该组织中的 LPO。另一方面，这两种元素单独和联合使用会破坏骨组织中的抗氧化防御机制和一些金属的内稳态，这可能导致成骨细胞和破骨细胞活动的平衡受到干扰，从而对骨骼健康产生负面影响。
