Usende Ifukibot L, Olopade James O, Emikpe Benjamin O, Oyagbemi Ademola A, Adedapo Adeolu A
Department of Veterinary Anatomy, University of Abuja, Nigeria.
Department of Veterinary Anatomy, University of Ibadan, Nigeria.
Int J Vet Sci Med. 2018 Mar 19;6(1):80-89. doi: 10.1016/j.ijvsm.2018.03.004. eCollection 2018 Jun.
Vanadium is a contaminant of crude oil that released into the atmosphere through burning of fossil fuels. The mechanism by which it exerts toxic influences had not been fully elucidated in African giant rat (AGR). This study investigates the mechanisms of sodium metavanadate (SMV) induced oxidative stress in AGR. A total of 24 adult male AGR weighing 600-850 g were used. Animals were randomly divided into six groups. Groups 1, 3 and 5 served as control while groups 2, 4 and 6 were treated with intraperitoneal 3 mg/kg body weight of SMV for 3, 7 and 14 days, respectively. Serum, brain, liver, testes, kidneys, spleen and lungs were harvested for biochemical assays. SMV induced significant increase in malondialdehyde, hydrogen peroxide, sulfhydryl (total thiol) and protein carbonyl levels but decreased non-protein thiol levels in tissues accessed. A significant decrease was observed in glutathione-S-transferase (GST), superoxide dismutase (SOD), reduced glutathione (GSH) and glutathione peroxidase (GPx) levels in SMV treated rats compared to controls. Serum myeloperoxidase, xanthine oxidase and Advanced Oxidative Protein Products (AOPP) were markedly increased while nitrous oxide levels were significantly decreased in all treated groups. SMV exposure to AGR induced oxidative stress through generation of reactive oxygen species (ROS) and depletion of the antioxidant defence system. These conditions could become severe with prolonged exposure.
钒是原油中的一种污染物，通过燃烧化石燃料释放到大气中。其在非洲巨鼠（AGR）中产生毒性影响的机制尚未完全阐明。本研究调查了偏钒酸钠（SMV）诱导AGR氧化应激的机制。总共使用了24只体重600 - 850克的成年雄性AGR。动物被随机分为六组。第1、3和5组作为对照组，而第2、4和6组分别腹腔注射3毫克/千克体重的SMV，持续3、7和14天。采集血清、脑、肝、睾丸、肾、脾和肺用于生化检测。SMV导致所检测组织中的丙二醛、过氧化氢、巯基（总硫醇）和蛋白质羰基水平显著升高，但非蛋白质硫醇水平降低。与对照组相比，SMV处理的大鼠中谷胱甘肽 - S - 转移酶（GST）、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）水平显著降低。所有处理组的血清髓过氧化物酶、黄嘌呤氧化酶和晚期氧化蛋白产物（AOPP）明显升高，而一氧化氮水平显著降低。SMV暴露于AGR会通过产生活性氧（ROS）和消耗抗氧化防御系统诱导氧化应激。长期暴露这些情况可能会变得严重。
