CTL14是在幼虫后期诱导产生的一种识别受体，可调节棉铃虫的抗真菌免疫。
CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera.
作者信息
Cheng Yang, Lin Zhe, Wang Ju-Mei, Xing Long-Sheng, Xiong Guang-Hua, Zou Zhen
机构信息
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
出版信息
Dev Comp Immunol. 2018 Jul;84:142-152. doi: 10.1016/j.dci.2018.02.010. Epub 2018 Feb 14.
C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was found to form aggregates with zymosan and B. bassiana in vitro. Immunoprecipitation studies demonstrated that CTL14 interacted with serine proteinases (SP), serine proteinase inhibitor (serpin), prophenoloxidases (PPO) and vitellogenin (Vg) in the larval hemolymph. Furthermore, depletion of CTL14 using dsRNA led to dramatic decrease in the expression level of PPO1. Additionally, CTL14 depleted H. armigera decreased the resistance to fungal challenge. Taken together, our study showed the direct involvement of CTL14 in the anti-fungal immunity of H. armigera, which further explained the stronger immune responses in the fifth instar compared to the second instar larvae.
C型凝集素（CTL）通常被认为是昆虫先天免疫中的模式识别受体。在此，我们发现棉铃虫的CTL14仅在五龄幼虫中被昆虫病原球孢白僵菌感染激活，而在二龄幼虫中未被激活。发现重组CTL14蛋白在体外与酵母聚糖和球孢白僵菌形成聚集体。免疫沉淀研究表明，CTL14与幼虫血淋巴中的丝氨酸蛋白酶（SP）、丝氨酸蛋白酶抑制剂（serpin）、前酚氧化酶（PPO）和卵黄原蛋白（Vg）相互作用。此外，使用dsRNA耗尽CTL14导致PPO1表达水平显著降低。另外，CTL14缺失的棉铃虫对真菌攻击的抗性降低。综上所述，我们的研究表明CTL14直接参与棉铃虫的抗真菌免疫，这进一步解释了五龄幼虫相比二龄幼虫具有更强的免疫反应。
Zotero 插件