• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-2055 通过脂肪酸合酶表达调节脂质代谢。

miR-2055 regulates lipid metabolism via fatty acid synthase expression.

机构信息

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.

College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, People's Republic of China.

出版信息

Open Biol. 2022 Mar;12(3):210307. doi: 10.1098/rsob.210307. Epub 2022 Mar 2.

DOI:10.1098/rsob.210307
PMID:35232249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8889172/
Abstract

Insect hormones and microRNAs regulate lipid metabolism, but the mechanisms are not fully elucidated. Here, we found that cotton bollworm larvae feeding on (AT) leaves had a lower triacylglycerol (TAG) level and more delayed development than individuals feeding on artificial diet (AD). Association analysis of small RNA and mRNA revealed that the level of miR-2055, a microRNA related to lipid metabolism, was significantly higher in larvae feeding on AT. Dual-luciferase reporter assays demonstrated miR-2055 binding to 3' UTR of fatty acid synthase (FAS) mRNA to suppress its expression. Elevating the level of miR-2055 in larvae by agomir injection decreased FAS mRNA and protein levels, which resulted in reduction of free fatty acid (FFA) and TAG in fat body. Interestingly, assays illustrated that juvenile hormone (JH) increased miR-2055 accumulation in a dosage-dependent manner, whereas knockdown of Methoprene tolerant (Met) or Kruppel homologue 1 (Kr-h1) decreased the miR-2055 level. This implied that JH induces the expression of miR-2055 via a Met-Kr-h1 signal. These findings demonstrate that JH and miRNA cooperate to modulate lipid synthesis, which provides new insights into the regulatory mechanisms of metabolism in insects.

摘要

昆虫激素和 microRNAs 调节脂质代谢，但机制尚未完全阐明。在这里，我们发现棉铃虫幼虫取食 (AT) 叶片比取食人工饲料 (AD) 的个体 TAG 水平更低，发育更延迟。小 RNA 和 mRNA 的关联分析表明，与脂质代谢相关的 microRNA miR-2055 在取食 AT 的幼虫中水平显著升高。双荧光素酶报告基因检测表明，miR-2055 结合到脂肪酸合酶 (FAS) mRNA 的 3'UTR 以抑制其表达。通过 agomir 注射提高幼虫中 miR-2055 的水平会降低 FAS mRNA 和蛋白水平，导致脂肪体中游离脂肪酸 (FFA) 和 TAG 减少。有趣的是， 实验表明，保幼激素 (JH) 以剂量依赖的方式增加 miR-2055 的积累，而 Methoprene tolerant (Met) 或 Kruppel 同源物 1 (Kr-h1) 的敲低会降低 miR-2055 水平。这表明 JH 通过 Met-Kr-h1 信号诱导 miR-2055 的表达。这些发现表明 JH 和 miRNA 合作调节脂质合成，为昆虫代谢的调控机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/556f1e44c8c8/rsob210307f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/b2fcb79b9372/rsob210307f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/c1c3015ead30/rsob210307f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/72e9c922333e/rsob210307f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/aaff4cbc7033/rsob210307f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/ca47aed13bc1/rsob210307f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/556f1e44c8c8/rsob210307f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/b2fcb79b9372/rsob210307f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/c1c3015ead30/rsob210307f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/72e9c922333e/rsob210307f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/aaff4cbc7033/rsob210307f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/ca47aed13bc1/rsob210307f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/8889172/556f1e44c8c8/rsob210307f06.jpg

相似文献

1
miR-2055 regulates lipid metabolism via fatty acid synthase expression.miR-2055 通过脂肪酸合酶表达调节脂质代谢。
Open Biol. 2022 Mar;12(3):210307. doi: 10.1098/rsob.210307. Epub 2022 Mar 2.
2
Juvenile hormone induces methoprene-tolerant 1 phosphorylation to increase interaction with Taiman in Helicoverpa armigera.保幼激素诱导灭幼脲耐受 1 磷酸化，增加与 Taiman 在棉铃虫中的相互作用。
Insect Biochem Mol Biol. 2021 Mar;130:103519. doi: 10.1016/j.ibmb.2021.103519. Epub 2021 Jan 13.
3
Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-signaling regulating the maturation of male accessory glands in the moth Agrotis ipsilon.保幼激素耐受性和 Krüppel 同源物 1 参与调控斜纹夜蛾雄附腺成熟的保幼激素信号。
Insect Biochem Mol Biol. 2021 May;132:103566. doi: 10.1016/j.ibmb.2021.103566. Epub 2021 Mar 17.
4
Methoprene-tolerant 1 regulates gene transcription to maintain insect larval status.耐甲氧普烯1调节基因转录以维持昆虫幼虫状态。
J Mol Endocrinol. 2014 Aug;53(1):93-104. doi: 10.1530/JME-14-0019. Epub 2014 May 28.
5
Dissecting the role of Krüppel homolog 1 in the metamorphosis and female reproduction of the cotton bollworm, Helicoverpa armigera.解析克氏同源盒基因1在棉铃虫变态发育及雌性生殖中的作用
Insect Mol Biol. 2018 Aug;27(4):492-504. doi: 10.1111/imb.12389. Epub 2018 May 2.
6
Lipases are differentially regulated by hormones to maintain free fatty acid homeostasis for insect brain development.脂肪酶受激素的差异调节，以维持昆虫大脑发育的游离脂肪酸动态平衡。
BMC Biol. 2024 Aug 13;22(1):171. doi: 10.1186/s12915-024-01973-3.
7
Synergistic action of the transcription factors Krüppel homolog 1 and Hairy in juvenile hormone/Methoprene-tolerant-mediated gene-repression in the mosquito Aedes aegypti.转录因子 Krüppel 同源物 1 和 Hairy 在蚊虫 Aedes aegypti 中共同作用于保幼激素/灭幼脲耐受介导的基因抑制。
PLoS Genet. 2019 Oct 29;15(10):e1008443. doi: 10.1371/journal.pgen.1008443. eCollection 2019 Oct.
8
regulates the development of and its cascade regulation pattern in the juvenile hormone signalling pathway.调控 和 其在保幼激素信号通路中的级联调控模式的发育。
Open Biol. 2023 May;13(5):220372. doi: 10.1098/rsob.220372. Epub 2023 May 31.
9
Heat shock protein 83 (Hsp83) facilitates methoprene-tolerant (Met) nuclear import to modulate juvenile hormone signaling.热休克蛋白83（Hsp83）促进耐甲氧普烯（Met）核输入以调节保幼激素信号传导。
J Biol Chem. 2014 Oct 3;289(40):27874-85. doi: 10.1074/jbc.M114.582825. Epub 2014 Aug 13.
10
MicroRNA let-7-5p targets the juvenile hormone primary response gene Krüppel homolog 1 and regulates reproductive diapause in Galeruca daurica.微小RNA let-7-5p靶向保幼激素初级反应基因Krüppel同源物1并调节兴安落叶松叶蜂的生殖滞育。
Insect Biochem Mol Biol. 2022 Mar;142:103727. doi: 10.1016/j.ibmb.2022.103727. Epub 2022 Jan 29.

引用本文的文献

1
Developmental Expression Patterns of miRNA in Walker (Lepidotera: Noctuidae).沃克蛾（鳞翅目：夜蛾科）中miRNA的发育表达模式
Genes (Basel). 2025 Feb 19;16(2):234. doi: 10.3390/genes16020234.
2
Knockdown of Impairs Fecundity by Inhibiting Lipid Accumulation and Increasing Glycogen Storage in .敲低……通过抑制……中的脂质积累和增加糖原储存来损害繁殖力。 （注：原文中“Knockdown of ”和“in ”后面的内容缺失，所以只能翻译到这种程度）
Insects. 2025 Jan 26;16(2):120. doi: 10.3390/insects16020120.
3
The accumulation of modular serine protease mediated by a novel circRNA sponging miRNA increases Aedes aegypti immunity to fungus.

本文引用的文献

1
Whitefly hijacks a plant detoxification gene that neutralizes plant toxins.粉虱劫持了一个能使植物毒素失活的植物解毒基因。
Cell. 2021 Apr 1;184(7):1693-1705.e17. doi: 10.1016/j.cell.2021.02.014. Epub 2021 Mar 25.
2
MicroRNA 182 is a Novel Negative Regulator of Adipogenesis by Targeting CCAAT/Enhancer-Binding Protein α.miRNA-182 是通过靶向 CCAAT/增强子结合蛋白α 来调控脂肪生成的新型负调控因子。
Obesity (Silver Spring). 2020 Aug;28(8):1467-1476. doi: 10.1002/oby.22863. Epub 2020 Jun 23.
3
Triacylglycerol Metabolism in .脂肪组织中甘油三酯代谢。
新型环状 RNA 通过海绵吸附 miRNA 来介导模块丝氨酸蛋白酶的积累，从而增强埃及伊蚊对真菌的免疫力。
BMC Biol. 2024 Jan 17;22(1):7. doi: 10.1186/s12915-024-01811-6.
4
Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport in .Wds 介导的 H3K4me3 修饰调节 中的脂质合成和运输。
Int J Mol Sci. 2023 Mar 24;24(7):6125. doi: 10.3390/ijms24076125.
5
Thermal Stress Induces Metabolic Responses in Juvenile Qingtian Paddy Field Carp .热应激诱导青田田鲤幼鱼的代谢反应
Animals (Basel). 2022 Dec 2;12(23):3395. doi: 10.3390/ani12233395.
6
The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, (Lepidoptera: Noctuidae).脂肪酸合酶在一种破坏性昆虫（鳞翅目：夜蛾科）的脂质代谢和变态发育中的关键作用。
Int J Mol Sci. 2022 Aug 13;23(16):9064. doi: 10.3390/ijms23169064.
Genetics. 2018 Dec;210(4):1163-1184. doi: 10.1534/genetics.118.301583.
4
Deficiency of Brummer Impaires Lipid Mobilization and JH-Mediated Vitellogenesis in the Brown Planthopper, .布鲁默蛋白缺乏会损害褐飞虱的脂质动员和保幼激素介导的卵黄发生 。
Front Physiol. 2018 Oct 30;9:1535. doi: 10.3389/fphys.2018.01535. eCollection 2018.
5
A Metalloprotease Homolog Venom Protein From a Parasitoid Wasp Suppresses the Toll Pathway in Host Hemocytes.一种金属蛋白酶同源物毒液蛋白来自寄生蜂抑制宿主血细胞中的 Toll 途径。
Front Immunol. 2018 Oct 23;9:2301. doi: 10.3389/fimmu.2018.02301. eCollection 2018.
6
Dominant point mutation in a tetraspanin gene associated with field-evolved resistance of cotton bollworm to transgenic Bt cotton.与田间棉铃虫对转 Bt 棉产生抗性相关的一个四跨膜蛋白基因的显性点突变。
Proc Natl Acad Sci U S A. 2018 Nov 13;115(46):11760-11765. doi: 10.1073/pnas.1812138115. Epub 2018 Oct 31.
7
Lipid metabolism in insect disease vectors.昆虫病媒的脂质代谢。
Insect Biochem Mol Biol. 2018 Oct;101:108-123. doi: 10.1016/j.ibmb.2018.08.005. Epub 2018 Aug 29.
8
CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera.CTL14是在幼虫后期诱导产生的一种识别受体，可调节棉铃虫的抗真菌免疫。
Dev Comp Immunol. 2018 Jul;84:142-152. doi: 10.1016/j.dci.2018.02.010. Epub 2018 Feb 14.
9
Endocrine and physiological regulation of neutral fat storage in Drosophila.果蝇中性脂肪储存的内分泌和生理调节。
Mol Cell Endocrinol. 2018 Feb 5;461:165-177. doi: 10.1016/j.mce.2017.09.008. Epub 2017 Sep 8.
10
MicroRNA-277 targets and to control lipid metabolism and reproduction in mosquitoes.miRNA-277 靶向 和 以控制脂质代谢和蚊子的繁殖。
Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):E8017-E8024. doi: 10.1073/pnas.1710970114. Epub 2017 Sep 5.