Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Cells. 2023 Feb 13;12(4):608. doi: 10.3390/cells12040608.
C-type lectin X (CTL-X) plays critical roles in immune defense, cell adhesion, and developmental regulation. Here, a transmembrane CTL-X of , with multiple domains was characterized. It was highly expressed in the early and late pupae and early adults and was distributed in all examined tissues. In addition, its expression levels were significantly induced after being challenged with pathogen-associated molecular patterns (PAMPs) and bacteria. In vitro, the recombinant TcCTL15 could recognize bacteria through binding PAMPs and exhibit agglutinating activity against a narrow range of bacteria in the presence of Ca. RNAi-mediated -knockdown-larvae infected with and showed less survival, had activated immune signaling pathways, and induced the expression of antimicrobial peptide genes. Moreover, silencing caused eclosion defects by impairing ecdysone and crustacean cardioactive peptide receptors (CCAPRs). Suppression of in female adults led to defects in ovary development and fecundity, accompanied by concomitant reductions in the mRNA levels of vitellogenin ( and farnesol dehydrogenase (. These findings imply that TcCTL15 has extensive functions in developmental regulation and antibacterial immunity. Uncovering the function of TcCTL15 will enrich the understanding of CTL-X in invertebrates. Its multiple biological functions endow the potential to be an attractive target for pest control.
C 型凝集素 X（CTL-X）在免疫防御、细胞黏附和发育调控中发挥着关键作用。在这里，我们对一种具有多个结构域的跨膜 CTL-X 进行了研究。它在早期和晚期蛹以及早期成虫中高度表达，并分布在所有检查的组织中。此外，其表达水平在受到病原体相关分子模式（PAMPs）和细菌的刺激后显著诱导。在体外，重组 TcCTL15 可以通过结合 PAMPs 识别细菌，并在 Ca 的存在下对窄范围的细菌表现出凝集活性。RNAi 介导的 -基因敲低幼虫感染 和 后存活率降低，激活了免疫信号通路，并诱导抗菌肽基因的表达。此外，沉默 会通过损害蜕皮激素和甲壳动物心活性肽受体（CCAPRs）导致蜕皮缺陷。在雌性成虫中抑制 会导致卵巢发育和生殖力缺陷，同时伴随着卵黄原蛋白（ 和法呢醇脱氢酶（ 的 mRNA 水平降低。这些发现表明 TcCTL15 在发育调控和抗菌免疫中具有广泛的功能。揭示 TcCTL15 的功能将丰富我们对无脊椎动物 CTL-X 的理解。其多种生物学功能使其成为害虫防治的有吸引力的目标。
