DNA Barcoding and Marine Genomics Laboratory, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
DNA Barcoding and Marine Genomics Laboratory, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Bioorg Chem. 2018 Apr;77:494-506. doi: 10.1016/j.bioorg.2018.02.014. Epub 2018 Feb 12.
The marine bacterial strain Bacillus cereus was used to produce amylase enzyme and has excellent alkali-stable and thermostable enzymatic activity. The combined effects of pH, temperature and incubation time on amylase activity were studied using response surface methodology. The amylase enzyme activity was also determined in the presence of various metal ions, chelating agents, detergents and the results showed that the maximum enzyme activity was observed in the presence of calcium chloride (96.1%), EDTA (63.4%) and surf excel (90.6%). The amylase enzyme exhibited excellent antibiofilm activity against marine derived biofilm forming bacteria Pseudomonas aeruginosa and Staphylococcus aureus in microtiter plate assay and congo red assay. Light and confocal laser scanning microscopic (CLSM) analysis were also used to confirm the potential biofilm activity of amylase enzyme. The CLSM analysis showed the inhibition of complete biofilm formation on amylase enzyme treated glass surface. Further in vivo toxicity analysis of amylase enzyme was determined against marine organisms Dioithona rigida and Artemia salina. The results showed that there is no morphological changes were observed due to the minimal toxicity of amylase enzyme. Overall these findings suggested that marine bacterial derived amylase enzyme could be developed as potential antibiofilm agent.
海洋细菌蜡状芽孢杆菌被用来生产淀粉酶，具有极好的碱性稳定性和热稳定性的酶活性。通过响应面法研究了 pH 值、温度和孵育时间对淀粉酶活性的综合影响。还测定了各种金属离子、螯合剂、洗涤剂存在下的淀粉酶活性，结果表明，在氯化钙（96.1%）、EDTA（63.4%）和 surf excel（90.6%）存在下观察到最大酶活性。在微量滴定板测定和刚果红测定中，淀粉酶对海洋来源的生物膜形成细菌铜绿假单胞菌和金黄色葡萄球菌表现出优异的抗生物膜活性。还使用荧光和共聚焦激光扫描显微镜（CLSM）分析来确认淀粉酶的潜在生物膜活性。CLSM 分析表明，在淀粉酶处理的玻璃表面上完全抑制了生物膜的形成。进一步对海洋生物旋口硅藻和卤虫进行了淀粉酶的体内毒性分析。结果表明，由于淀粉酶的最小毒性，没有观察到形态变化。总的来说，这些发现表明，海洋细菌来源的淀粉酶可以开发为潜在的抗生物膜剂。
