Department of Biotechnology, University of Engineering & Management, Kolkata, India.
Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata, India.
Appl Biochem Biotechnol. 2021 Jun;193(6):1853-1872. doi: 10.1007/s12010-021-03509-9. Epub 2021 Mar 1.
α-amylase is known to have antibiofilm activity against biofilms of both Gram positive and Gram-negative bacterial strains. Partially purified α-amylase from Bacillus subtilis was found to have inhibit biofilm formed by P. aeruginosa and S. aureus. The spectrophotometric and microscopic studies revealed that the antibiofilm efficacy of the working strain is greater than commercially purchased α-amylase. Response surface methodology (RSM) and artificial neural network (ANN) help to predict the optimum conditions [pH 8, treatment time 6 h and enzyme concentration (200 µg/mL)] for maximum biofilm eradication. This was confirmed by several in vitro experiments. Molecular docking interactions of α-amylase with the extracellular polymeric substances (EPS) of both P. aeruginosa and S. aureus indicate towards the existence of an efficient energy driven spontaneous process. Thus, this study highlights a combination of experimental and computational approach showing the naturally extracted α-amylase from B. subtilis having the potency of removing the biofilms of harmful bacterial strains involved in causing various nosocomial infections.
α-淀粉酶已知对革兰氏阳性和革兰氏阴性菌的生物膜具有抗生物膜活性。从枯草芽孢杆菌中分离出的部分纯化α-淀粉酶被发现能抑制铜绿假单胞菌和金黄色葡萄球菌形成的生物膜。分光光度法和显微镜研究表明，工作菌株的抗生物膜效果大于市售的α-淀粉酶。响应面法（RSM）和人工神经网络（ANN）有助于预测最佳条件[pH8、处理时间 6 小时和酶浓度（200μg/mL）]，以实现最大的生物膜清除。这通过几项体外实验得到了证实。α-淀粉酶与铜绿假单胞菌和金黄色葡萄球菌的细胞外聚合物（EPS）的分子对接相互作用表明，存在一种有效的能量驱动的自发过程。因此，这项研究强调了实验和计算方法的结合，表明从枯草芽孢杆菌中天然提取的α-淀粉酶具有去除引起各种医院感染的有害细菌菌株生物膜的能力。
