Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), University of Blida, 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria; National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismail 42415, Tipaza, Algeria.
Laboratory of Environmental Bioprocesses (LEBP), LMI COSYS-Med, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia.
Ecotoxicol Environ Saf. 2018 Jun 15;154:100-107. doi: 10.1016/j.ecoenv.2018.02.032. Epub 2018 Feb 15.
This study aims to investigate the ability of a biosurfactant produced by Marinobacter hydrocarbonoclasticus strain SdK644 isolated from hydrocarbon contaminated sediment to enhance the solubilization rate of crude oil contaminated seawater. Phylogenetic analysis shows that strain SdK644 was very closely related to M. hydrocarbonoclasticus with 16S rRNA gene sequence similarity of 97.44%. Using waste frying oil as inducer carbon source, the producing biosurfactant by strain SdK644 was applied to improve crude oil solubilization in seawater. The preliminary characterization of the produced biosurfactant by FT-IR analysis indicates its possible classification in a glycolipids group. Results from crude oil solubilization assay showed that SdK644 strain biosurfactant was 2-fold greater than Tween 80 surfactant in crude oil solubilization and 12-fold higher than seawater control, as shown by GC-MS analysis of aliphatic compounds. Furthermore, this bioactive compound was shown to be nontoxic against Artemia larvae in short-term acute toxicity bioassay. Generally, the results showed the possible use of M. hydrocarbonoclasticus strain SdK644 biosurfactant in bioremediation processes of the marine environments.
本研究旨在探讨从烃类污染沉积物中分离得到的海杆菌（Marinobacter hydrocarbonoclasticus）菌株 SdK644 产生的生物表面活性剂提高被原油污染海水的增溶率的能力。系统发育分析表明，菌株 SdK644 与海杆菌的 16S rRNA 基因序列相似性为 97.44%。利用废食用油作为诱导碳源，应用菌株 SdK644 产生的生物表面活性剂来提高原油在海水中的增溶率。通过傅里叶变换红外（FT-IR）分析对产生的生物表面活性剂进行初步表征，表明其可能属于糖脂类。原油增溶实验结果表明，SdK644 菌株生物表面活性剂在原油增溶方面比吐温 80 表面活性剂高 2 倍，比海水对照组高 12 倍，这通过气相色谱-质谱（GC-MS）分析脂肪族化合物得到证实。此外，该生物活性化合物在短期急性毒性生物测定中对卤虫幼虫无毒性。总的来说，结果表明海杆菌（Marinobacter hydrocarbonoclasticus）菌株 SdK644 生物表面活性剂在海洋环境生物修复过程中有潜在的应用。
