在秘鲁嗜阿米巴（变形虫纲；嗜阿米巴科）中共培养的鲑鱼辛那米衣原体（衣原体目；辛那米科）的基因分型。
Genotyping of Candidatus Syngnamydia salmonis (chlamydiales; Simkaniaceae) co-cultured in Paramoeba perurans (amoebozoa; Paramoebidae).
作者信息
Nylund Are, Pistone Dario, Trösse Christiane, Blindheim Steffen, Andersen Linda, Plarre Heidrun
机构信息
Department of Biology, University of Bergen, Thormohlensgt 55, 5020, Bergen, Norway.
The Industrial and Aquatic Laboratory (ILAB), Thormohlensgt 55, 5020, Bergen, Norway.
出版信息
Arch Microbiol. 2018 Aug;200(6):859-867. doi: 10.1007/s00203-018-1488-0. Epub 2018 Feb 17.
Candidatus Syngnamydia salmonis (Chlamydiales, Simkaniaceae) was described as an epitheliocystis-causing bacterium from the gills of Atlantic salmon (Salmo salar) in Norway. A bacterium showing 99.2% 16S rRNA identity to Cand. S. salmonis is able to multiply in Paramoeba perurans and based on the classification criteria this bacterium could represent the same species as Cand. S. salmonis. Sequencing the genome of the cultured bacterium has made it possible to fulfill the minimal standards for genetic characterization of species within the order Chlamydiales. The complete rRNA genes, the amino acid sequences of SucA, PepF, Adk, HemL, DnaA, FtsK and FabI, are presented in addition to the morphology of the Chlamydia-like morphs in the cytoplasm of P. perurans.
“鲑鱼辛那米衣原体（衣原体目，辛卡尼亚科）”最初是在挪威从大西洋鲑（Salmo salar）的鳃中分离出来的一种引起上皮囊肿的细菌。一种与“鲑鱼辛那米衣原体”16S rRNA序列相似度达99.2%的细菌能够在秘鲁嗜阿米巴（Paramoeba perurans）中繁殖，根据分类标准，该细菌可能与“鲑鱼辛那米衣原体”属于同一物种。对培养细菌的基因组进行测序，使得满足衣原体目物种遗传特征描述的最低标准成为可能。除了秘鲁嗜阿米巴细胞质中衣原体样形态的形态学特征外，还展示了完整的rRNA基因以及SucA、PepF、Adk、HemL、DnaA、FtsK和FabI的氨基酸序列。
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