Bou Khalil Jacques Y, Benamar Samia, Baudoin Jean-Pierre, Croce Olivier, Blanc-Tailleur Caroline, Pagnier Isabelle, Raoult Didier, La Scola Bernard
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Facultés de Médecine et de Pharmacie, UM63 Centre National de la Recherche Scientifique 7278 IRD 198 Institut National de la Santé et de la Recherche Médicale U1095, Aix-Marseille Université Marseille, France.
Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Institut Hospitalo-Universitaire Méditerranée Infection, Assistance Publique - Hôpitaux de Marseille Marseille, France.
Front Cell Infect Microbiol. 2016 Mar 15;6:31. doi: 10.3389/fcimb.2016.00031. eCollection 2016.
The study of amoeba-associated Chlamydiae is a dynamic field in which new species are increasingly reported. In the present work, we characterized the developmental cycle and analyzed the genome of a new member of this group associated with Vermamoeba vermiformis, we propose to name "Rubidus massiliensis." This bacterium is well-adapted to its amoeba host and do not reside inside of inclusion vacuoles after phagocytosis. It has a developmental cycle typical of this family of bacteria, with a transition from condensed elementary bodies to hypodense replicative reticulate bodies. Multiplication occurs through binary fission of the reticulate bodies. The genome of "R. massiliensis" consists of a 2.8 Mbp chromosome and two plasmids (pRm1, pRm2) consisting of 39,075 bp and 80,897 bp, respectively, a feature that is unique within this group. The Re-analysis of the Chlamydiales genomes including the one of "R. massiliensis" slightly modified the previous phylogeny of the tlc gene encoding the ADP/ATP translocase. Our analysis suggested that the tlc gene could have been transferred to plant and algal plastids before the transfer to Rickettsiales, and that this gene was probably duplicated several times.
对与变形虫相关的衣原体的研究是一个充满活力的领域，新物种的报道越来越多。在本研究中，我们对与蠕虫状变形虫相关的该类群新成员的发育周期进行了表征，并分析了其基因组，我们提议将其命名为“马赛鲁比杜斯（Rubidus massiliensis）”。这种细菌很好地适应了其变形虫宿主，吞噬后并不存在于包涵体内。它具有该细菌家族典型的发育周期，从浓缩的原体过渡到低密度的复制型网状体。通过网状体的二分裂进行繁殖。“马赛鲁比杜斯”的基因组由一条280万碱基对的染色体和两个质粒（pRm1、pRm2）组成，分别为39075碱基对和80897碱基对，这一特征在该类群中是独一无二的。对包括“马赛鲁比杜斯”在内的衣原体基因组的重新分析略微修改了先前编码ADP/ATP转位酶的tlc基因的系统发育。我们的分析表明，tlc基因可能在转移到立克次氏体之前就已转移到植物和藻类质体中，并且该基因可能被复制了几次。
