Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysuru, 570 020, Karnataka, India.
Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysuru, 570 020, Karnataka, India.
Carbohydr Polym. 2018 Apr 15;186:402-410. doi: 10.1016/j.carbpol.2018.01.053. Epub 2018 Jan 31.
Swallow root pectic oligosaccharide fraction (SRO1) from swallow root pectic polysaccharide (SRPP) possessed a molecular size of 831 Da. Structural analysis revealed that it is a rhamnogalacturonan I type, bearing arabinogalactan side chain with β-d-(1→4) galactose along with α-l-Araf (1→5)-α-l-Araf (1→3) structure on α-d-GalA-OAc-(1→2)-α-l-Rha-(1→4)- linear backbone. β-d (1→4) linked galactose being the specific sugar for galectin-3, SRO1 had potentials in inhibiting galectin-3 mediated cancer progression. SRO1 inhibited galectin-3 mediated agglutination, in vitro, effectively with MIC of 1.08 μg/ mL and down regulated mRNA levels of galectin-3 (∼92%) along with its downstream key protein that inhibits apoptosis - survivin (∼78%) suggesting the capability of SRO1 in inhibiting galectin-3 mediated cancer promoting pathway. This is the first report, which highlights the inhibition of interplay of galectin-3 and survivin by a dietary pectic oligosaccharide.
来自蒲公英果胶多糖（SRPP）的 swallow 根果胶低聚糖部分（SRO1）具有 831Da 的分子量。结构分析表明，它是鼠李半乳糖醛酸 I 型，带有阿拉伯半乳聚糖侧链，β-d-（1→4）半乳糖与α-l-Araf（1→5）-α-l-Araf（1→3）结构在α-d-GalA-OAc-（1→2）-α-l-Rha-（1→4）-线性主链上。β-d（1→4）连接的半乳糖是半乳糖凝集素-3 的特异性糖，SRO1 具有抑制半乳糖凝集素-3 介导的癌症进展的潜力。SRO1 有效抑制了半乳糖凝集素-3 介导的体外凝集，MIC 为 1.08μg/ml，并下调了半乳糖凝集素-3（约 92%）及其下游抑制细胞凋亡的关键蛋白 - 存活素（约 78%）的 mRNA 水平，表明 SRO1 具有抑制半乳糖凝集素-3 介导的促进癌症的途径的能力。这是第一个报道，强调了饮食果胶低聚糖对半乳糖凝集素-3 和存活素相互作用的抑制作用。
