Jones Corinne A, Duffy Mary K, Hoffman Sarah A, Schultz-Darken Nancy J, Braun Katarina M, Ciucci Michelle R, Emborg Marina E
a Neuroscience Training Program, University of Wisconsin-Madison , Madison , WI , USA.
b Wisconsin National Primate Research Center , University of Wisconsin-Madison , Madison , WI , USA.
Neurol Res. 2018 Apr;40(4):303-311. doi: 10.1080/01616412.2018.1438226. Epub 2018 Feb 19.
Objectives In order to facilitate the study of vocalizations in emerging genetic common marmoset models of neurodegenerative disorders, we aimed to analyze call-type changes across age in a translational research environment. We hypothesized that acoustic parameters of vocalizations would change with age, reflecting growth of the vocal apparatus and a maturation of control needed to make adult-like calls. Methods Nineteen developing common marmosets were longitudinally video- and audio-recorded between the ages of 1-149 days in a naturalistic setting without any vocalization elicitation protocol. Vocalizations were coded for call type (cry, tsik, trill, phee, and trill-phee) and analyzed for duration (sec), minimum and maximum frequency (Hz), and bandwidth (Hz). Mixed model linear regressions were performed to assess the effects of age on call parameters listed above for each call type. Results Cries decreased in duration (P = 0.038), maximum frequency (P = 0.047), and bandwidth (P = 0.023) with age. Tsik calls decreased in duration (P = 0.002) and increased in minimum frequency (P = 0.004) and maximum frequency (P = 0.005) with age. Trill calls increased in duration (P = 0.003), and trillphee bandwidth (P = 0.031) decreased with age. Discussion Our results demonstrate that development of common marmoset vocalizations is call type dependent and that changes in acoustic parameters can be detected without complex vocalization elicitation paradigms or specialized audio recording equipment. Thus, we demonstrate the feasibility of a naturalistic protocol to collect and objectively analyze marmoset vocalizations longitudinally. This approach may be useful for studying vocal communication deficits in genetic models of neurodegenerative disorders.
目的 为了促进在新兴的神经退行性疾病基因普通狨猴模型中对发声的研究，我们旨在分析在转化研究环境中不同年龄阶段的叫声类型变化。我们假设发声的声学参数会随年龄变化，反映发声器官的生长以及发出类似成年叫声所需控制能力的成熟。方法 在自然环境中，对19只发育中的普通狨猴在1至149日龄期间进行纵向视频和音频记录，无需任何发声诱发方案。对发声进行叫声类型编码（哭声、tsik声、颤音、phee声和颤音 - phee声），并分析其持续时间（秒）、最低和最高频率（赫兹）以及带宽（赫兹）。进行混合模型线性回归，以评估年龄对上述每种叫声类型的叫声参数的影响。结果 哭声的持续时间（P = 0.038）、最高频率（P = 0.047）和带宽（P = 0.023）随年龄增长而降低。Tsik叫声的持续时间（P = 0.002）随年龄增长而降低，最低频率（P = 0.004）和最高频率（P = 0.005）随年龄增长而增加。颤音的持续时间（P = 0.003）随年龄增长而增加，颤音 - phee声的带宽（P = 0.031）随年龄增长而降低。讨论 我们的结果表明，普通狨猴发声的发育取决于叫声类型，并且无需复杂的发声诱发范式或专门的音频记录设备即可检测到声学参数的变化。因此，我们证明了一种自然方案用于纵向收集和客观分析狨猴发声的可行性。这种方法可能有助于研究神经退行性疾病基因模型中的发声交流缺陷。
