正常人血清可恢复免疫复合物阻断的人单核细胞中Fcγ受体的表达。
Normal human serum restores the expression of Fc gamma receptors in immune complex-blocked human mononuclear cells.
作者信息
Geffner J R, Serebrinsky G, Isturiz M A
出版信息
Immunology. 1986 Oct;59(2):239-43.
A progressive inhibition of both antibody-dependent cellular cytotoxicity (ADCC) and Fc gamma R expression was observed when peripheral blood mononuclear cells (PBMC) were incubated with soluble immune complexes (IC) at 37 degrees. Treatment of these IC-blocked PBMC with normal human serum (NHS) reversed both effects and increased the release of attached IC from the cell surface when compared to heat-inactivated serum (HI-NHS) or culture medium (TCM) treatments. Immunofluorescence studies demonstrated a redistribution of IC, predominantly in the form of caps in IC-blocked cells treated with HI-NHS (56%) or TCM (51%). However, when these IC-blocked cells were treated with NHS, only 11% of them showed caps redistribution. We propose that NHS regulation of Fc gamma R expression may be a physiological way of modulating the different immunological events triggered by IC.
当外周血单个核细胞（PBMC）在37℃下与可溶性免疫复合物（IC）孵育时，观察到抗体依赖性细胞毒性（ADCC）和FcγR表达均出现进行性抑制。与热灭活血清（HI-NHS）或培养基（TCM）处理相比，用正常人血清（NHS）处理这些被IC阻断的PBMC可逆转这两种效应，并增加细胞表面附着的IC的释放。免疫荧光研究表明IC发生了重新分布，在用HI-NHS（56%）或TCM（51%）处理的IC阻断细胞中，主要以帽状形式存在。然而，当用NHS处理这些被IC阻断的细胞时，只有11%的细胞显示帽状重新分布。我们提出，NHS对FcγR表达的调节可能是一种调节由IC触发的不同免疫事件的生理方式。
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