免疫复合物从淋巴细胞膜上的补体依赖性释放。
Complement-dependent release of immune complexes from the lymphocyte membrane.
作者信息
Miller G W, Saluk P H, Nussenzweig V
出版信息
J Exp Med. 1973 Sep 1;138(3):495-507. doi: 10.1084/jem.138.3.495.
Soluble antigen-antibody-complement complexes bound to mouse B lymphocytes are rapidly released from the cell membrane in the presence of normal serum from several mammalian species. The release is not the result of antigen-antibody dissociation or extensive degradation of the complexes. However, the released complexes have been altered because they will no longer bind to fresh lymphocytes. The release is not the result of lymphocyte damage mediated by complement. It is complement-dependent, and is generated either preferentially or exclusively via the alternate pathway, since it occurs in C4-deficient serum, is Mg(++) but not Ca(++) dependent, and requires C3 proactivator. C3 inactivator is not involved. The release activity of the serum, once generated, is unstable at 37 degrees C. The release of complexes from the lymphocyte membrane by serum provides a convenient assay for the functioning of the alternate pathway in the mouse and in other species.
与小鼠B淋巴细胞结合的可溶性抗原-抗体-补体复合物，在存在几种哺乳动物物种的正常血清时，会迅速从细胞膜上释放出来。这种释放不是抗原-抗体解离或复合物广泛降解的结果。然而，释放出的复合物已发生改变，因为它们不再能与新鲜淋巴细胞结合。这种释放不是补体介导的淋巴细胞损伤的结果。它是补体依赖性的，并且优先或仅通过替代途径产生，因为它发生在C4缺陷血清中，依赖Mg(++)而不是Ca(++)，并且需要C3前激活物。不涉及C3灭活剂。血清的释放活性一旦产生，在37℃时不稳定。血清使复合物从淋巴细胞膜上释放，为小鼠和其他物种中替代途径的功能提供了一种便捷的检测方法。
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