Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea.
Plant Proteomics Group, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Proteomics. 2018 Apr;18(7):e1700366. doi: 10.1002/pmic.201700366. Epub 2018 Mar 7.
Phytohormones are central to plant growth and development. Despite the advancement in our knowledge of hormone signaling, downstream targets, and their interactions upon hormones action remain largely fragmented, especially at the protein and metabolite levels. With an aim to get new insight into the effects of two hormones, ethylene (ET) and abscisic acid (ABA), this study utilizes an integrated proteomics and metabolomics approach to investigate their individual and combined (ABA+ET) signaling in soybean leaves. Targeting low-abundance proteins, our previously established protamine sulfate precipitation method was applied, followed by label-free quantification of identified proteins. A total of 4129 unique protein groups including 1083 differentially modulated in one (individual) or other (combined) treatments were discerned. Functional annotation of the identified proteins showed an increased abundance of proteins related to the flavonoid and isoflavonoid biosynthesis and MAPK signaling pathway in response to ET treatment. HPLC analysis showed an accumulation of isoflavones (genistin, daidzein, and genistein) upon ET treatment, in agreement with the proteomics results. A metabolome analysis assigned 79 metabolites and further confirmed the accumulation of flavonoids and isoflavonoids in response to ET. A potential cross-talk between ET and MAPK signaling, leading to the accumulation of flavonoids and isoflavonoids in soybean leaves is suggested.
植物激素是植物生长和发育的核心。尽管我们在激素信号转导、下游靶标及其在激素作用下的相互作用方面的知识有所进步，但在蛋白质和代谢物水平上，这些信息仍然非常分散。本研究旨在深入了解两种激素——乙烯（ET）和脱落酸（ABA）的作用，采用整合的蛋白质组学和代谢组学方法研究它们在大豆叶片中的单独和联合（ABA+ET）信号转导。针对低丰度蛋白质，我们应用了先前建立的鱼精蛋白硫酸盐沉淀法，然后对鉴定出的蛋白质进行无标记定量。总共鉴定出 4129 个独特的蛋白质组，其中 1083 个在一种（单独）或另一种（联合）处理中被差异调节。鉴定出的蛋白质的功能注释显示，对 ET 处理的反应中，与类黄酮和异黄酮生物合成以及 MAPK 信号通路相关的蛋白质丰度增加。HPLC 分析显示 ET 处理后异黄酮（染料木苷、大豆苷和染料木素）的积累，与蛋白质组学结果一致。代谢组学分析分配了 79 种代谢物，并进一步证实了对 ET 的反应中类黄酮和异黄酮的积累。建议 ET 和 MAPK 信号之间存在潜在的串扰，导致大豆叶片中类黄酮和异黄酮的积累。
