Barazetti André Riedi, Dealis Mickely Liuti, Basso Kawany Roque, Silva Maria Clara Davis, Alves Leonardo da Cruz, Parra Maria Eugênia Alcântara, Simionato Ane Stéfano, Cely Martha Viviana Torres, Macedo Arthur Ladeira, Silva Denise Brentan, Andrade Galdino
Microbial Ecology Laboratory, Department of Microbiology, Universidade Estadual de Londrina, Londrina 86057-970, PR, Brazil.
Agricultural and Environmental Sciences Institute, Federal University of Mato Grosso, Sinop 78550-728, MT, Brazil.
Microorganisms. 2024 Aug 2;12(8):1576. doi: 10.3390/microorganisms12081576.
are known as higher producers of secondary metabolites with antimicrobial properties and plant growth promoters, including resistance induction. These mechanisms should be an alternative to pesticide use in crop production. causes Asian soybean rust, representing a high loss of yield around the world. The objective of this paper was to evaluate the application of secondary metabolites produced by LV strain from the semi-purified fraction F4A in soybean plants to induce plant resistance against in field conditions. The experimental design was performed in randomized blocks with three replicates using two F4A doses (1 and 10 μg mL) combined or not with fungicides (Unizeb Gold or Sphere Max). The control treatment, with Uni + Sph, saponins, flavonoids, and sphingolipids, showed higher intensities in the plants. In contrast, plants treated with the F4A fraction mainly exhibited fatty acid derivatives and some non-identified compounds with nitrogen. Plants treated with Sphere Max, with or without F4A10, showed higher intensities of glycosylated flavonoids, such as kaempferol, luteolin, narigenin, and apigenin. Plants treated with F4A showed higher intensities of genistein and fatty acid derivatives. These increases in flavonoid compound biosynthesis and antioxidant properties probably contribute to the protection against reactive oxygen species (ROS).
被认为是具有抗菌特性的次生代谢产物的高产生产者以及植物生长促进剂，包括抗性诱导。这些机制应该是作物生产中农药使用的一种替代方法。 引发亚洲大豆锈病，在全球范围内造成高额产量损失。本文的目的是评估来自半纯化级分F4A的LV菌株产生的次生代谢产物在大豆植株中的应用，以在田间条件下诱导植物对 的抗性。实验设计采用随机区组，重复三次，使用两种F4A剂量（1和10μg/mL），并与杀菌剂（Unizeb Gold或Sphere Max）联合或不联合使用。对照处理，即Uni + Sph、皂苷、类黄酮和鞘脂，在植株中显示出更高的强度。相比之下，用F4A级分处理的植株主要表现出脂肪酸衍生物和一些含氮的未鉴定化合物。用Sphere Max处理的植株，无论有无F4A10，都显示出更高强度的糖基化类黄酮，如槲皮素、木犀草素、柚皮素和芹菜素。用F4A处理的植株显示出更高强度的染料木黄酮和脂肪酸衍生物。类黄酮化合物生物合成和抗氧化特性的这些增加可能有助于抵御活性氧（ROS）。
