Ackah Michael, Shi Yisu, Wu Mengmeng, Wang Lei, Guo Peng, Guo Liangliang, Jin Xin, Li Shaocong, Zhang Qiaonan, Qiu Changyu, Lin Qiang, Zhao Weiguo
School of Biology and Technology, Jiangsu University of Science and Technology, Sibaidu, Zhenjiang 212018, China.
Sericulture Research Institute, Guangxi Zhuang Autonomous Region, Nanning 530007, China.
Plants (Basel). 2021 Aug 9;10(8):1636. doi: 10.3390/plants10081636.
Mulberry is an economically significant crop for the sericulture industry worldwide. Stresses such as drought exposure have a significant influence on plant survival. Because metabolome directly reflects plant physiological condition, performing a global metabolomic analysis is one technique to examine this influence. Using a liquid chromatography-mass spectrometry (LC-MS) technique based on an untargeted metabolomic approach, the effect of drought stress on mulberry Yu-711 metabolic balance was examined. For this objective, Yu-711 leaves were subjected to two weeks of drought stress treatment and control without drought stress. Numerous differentially accumulated metabolic components in response to drought stress treatment were revealed by multivariate and univariate statistical analysis. Drought stress treatment (EG) revealed a more differentiated metabolite response than the control (CK). We found that the levels of total lipids, galactolipids, and phospholipids (PC, PA, PE) were significantly altered, producing 48% of the total differentially expressed metabolites. Fatty acyls components were the most abundant lipids expressed and decreased considerably by 73.6%. On the other hand, the prenol lipids class of lipids increased in drought leaves. Other classes of metabolites, including polyphenols (flavonoids and cinnamic acid), organic acid (amino acids), carbohydrates, benzenoids, and organoheterocyclic, had a dynamic trend in response to the drought stress. However, their levels under drought stress decreased significantly compared to the control. These findings give an overview for the understanding of global plant metabolic changes in defense mechanisms by revealing the mulberry plant metabolic profile through differentially accumulated compounds.
桑树是全球养蚕业中具有重要经济意义的作物。干旱等胁迫对植物的存活有重大影响。由于代谢组直接反映植物的生理状况，进行全面的代谢组学分析是研究这种影响的一种技术。采用基于非靶向代谢组学方法的液相色谱 - 质谱联用（LC - MS）技术，研究了干旱胁迫对桑树育711代谢平衡的影响。为此，对育711叶片进行了两周的干旱胁迫处理，并设置了无干旱胁迫的对照。通过多变量和单变量统计分析揭示了许多响应干旱胁迫处理而差异积累的代谢成分。干旱胁迫处理组（EG）显示出比对照组（CK）更明显的代谢物响应差异。我们发现总脂、半乳糖脂和磷脂（PC、PA、PE）的水平发生了显著变化，产生了48%的总差异表达代谢物。脂肪酰基成分是表达最丰富的脂质，且大幅下降了73.6%。另一方面，干旱叶片中异戊二烯脂质类别的脂质增加。其他类别的代谢物，包括多酚（黄酮类和肉桂酸）、有机酸（氨基酸）、碳水化合物、苯类化合物和有机杂环化合物，对干旱胁迫有动态响应趋势。然而，与对照相比，它们在干旱胁迫下的水平显著下降。这些发现通过差异积累的化合物揭示了桑树植物的代谢谱，为理解全球植物在防御机制中的代谢变化提供了一个概述。
