Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
Department of Comparative Biosciences, University of Illinois, Urbana, IL, United States.
Reprod Toxicol. 2018 Apr;77:70-79. doi: 10.1016/j.reprotox.2018.01.006. Epub 2018 Feb 16.
Di-(2-ethylhexyl) phthalate (DEHP) is an endocrine-disrupting chemical that has been shown to impair normal reproductive function in males and females. This study investigated whether adult exposure to environmental and occupational doses of DEHP alters homeostasis of uterine proliferation, morphology, and number of uterine glands. Adult female CD1 mice were orally dosed with DEHP (0, 20 μg/kg/day, 200 μg/kg/day, 20 mg/kg/day or 200 mg/kg/day) for 30 days. Results indicated that DEHP at 200 μg/kg/day caused a reduction in epithelial cell proliferation in the uterus (p < .05). We also observed an increase (p < .05) in the number of uterine glands in mice dosed with 200 mg/kg/day DEHP. Results showed that DEHP caused an increase (p < .05) in dilated blood vessels in the endometrium at 200 μg/kg/day, 20 mg/kg/day and 200 mg/kg/day. DEHP also increased proliferation of endometrial stromal cells at 200 μg/kg/day DEHP (p < .0010), 20 mg/kg/day DEHP (p < .0001) and 200 mg/kg/day DEHP (p < .0186). Results suggest that, exposure to specific doses of DEHP for 30 days can have adverse effects on reproductive function.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种内分泌干扰化学物质，已被证明会损害男性和女性的正常生殖功能。本研究调查了成年暴露于环境和职业剂量的 DEHP 是否会改变子宫增殖、形态和子宫腺数量的内稳态。成年雌性 CD1 小鼠经口给予 DEHP（0、20μg/kg/天、200μg/kg/天、20mg/kg/天或 200mg/kg/天）30 天。结果表明，200μg/kg/天的 DEHP 导致子宫上皮细胞增殖减少（p<.05）。我们还观察到 200mg/kg/天 DEHP 处理的小鼠子宫腺数量增加（p<.05）。结果表明，DEHP 导致子宫内膜 200μg/kg/天、20mg/kg/天和 200mg/kg/天的血管扩张（p<.05）。DEHP 还增加了 200μg/kg/天 DEHP（p<.0010）、20mg/kg/天 DEHP（p<.0001）和 200mg/kg/天 DEHP（p<.0186）的子宫内膜基质细胞增殖。结果表明，暴露于特定剂量的 DEHP 30 天可能对生殖功能产生不良影响。
