Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL, United States; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Reprod Toxicol. 2020 Apr;93:28-42. doi: 10.1016/j.reprotox.2019.12.006. Epub 2020 Jan 2.
Di(2-ethylhexyl) phthalate (DEHP) is a known endocrine disruptor and diisononyl phthalate (DiNP) is a common DEHP replacement chemical. However, little is known about late-life consequences due to DEHP or DiNP exposure during adulthood. Thus, this study tested the hypothesis that adult exposure to DEHP or DiNP affects female reproductive parameters during late-life in female mice. Female CD-1 mice (age 39-40 days) were dosed with either vehicle control, DEHP (20 μg/kg/day-200 mg/kg/day), or DiNP (20 μg/kg/day-200 mg/kg/day) for 10 days and breeding trials were conducted at 12 and 15 months post-dosing. Further, ovaries and sera were collected at 12, 15, and 18 months post-dosing. DEHP and DiNP disrupted estrous cyclicity, increased pregnancy loss, decreased fertility, altered the sex ratio of pups, altered ovarian follicle populations, and disrupted hormone levels. Collectively, these data show that short-term exposure to DEHP or DiNP during adulthood has long-term consequences in late-life.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种已知的内分泌干扰物，而邻苯二甲酸二异壬酯（DiNP）是一种常见的 DEHP 替代化学物质。然而，由于成年期暴露于 DEHP 或 DiNP 而导致的后期生命后果知之甚少。因此，本研究检验了这样一个假设，即成年期暴露于 DEHP 或 DiNP 会影响雌性小鼠后期生命中的雌性生殖参数。将 CD-1 雌性小鼠（39-40 天大）用 vehicle control、DEHP（20μg/kg/天-200mg/kg/天）或 DiNP（20μg/kg/天-200mg/kg/天）处理 10 天，并在给药后 12 和 15 个月进行繁殖试验。此外，在给药后 12、15 和 18 个月收集卵巢和血清。DEHP 和 DiNP 破坏动情周期，增加妊娠丢失，降低生育力，改变幼崽的性别比例，改变卵巢卵泡群，并扰乱激素水平。总之，这些数据表明，成年期短期暴露于 DEHP 或 DiNP 会对后期生命产生长期影响。
