Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg. FSB-101, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture, 277-8562, Japan.
AIST-UTokyo Advanced Operando-Measurement Technology Open Innovation Laboratory (OPERANDO-OIL), National Institute of Advanced Industrial Science and Technology (AIST), Bldg. Kashiwa Research Complex 2, 5-1-5 Kahiwanoha, Kashiwa, Chiba Prefecture, 277-8565, Japan.
BMC Genomics. 2018 Feb 20;19(1):149. doi: 10.1186/s12864-018-4526-z.
The size of the phenotypic effect of a gene has been thoroughly investigated in terms of fitness and specific morphological traits in the budding yeast Saccharomyces cerevisiae, but little is known about gross morphological abnormalities.
We identified 1126 holistic morphological effectors that cause severe gross morphological abnormality when deleted, and 2241 specific morphological effectors with weak holistic effects but distinctive effects on yeast morphology. Holistic effectors fell into many gene function categories and acted as network hubs, affecting a large number of morphological traits, interacting with a large number of genes, and facilitating high protein expression. Holistic morphological abnormality was useful for estimating the importance of a gene to morphology. The contribution of gene importance to fitness and morphology could be used to efficiently classify genes into functional groups.
Holistic morphological abnormality can be used as a reproducible and reliable gene feature for high-dimensional morphological phenotyping. It can be used in many functional genomic applications.
在酿酒酵母（Saccharomyces cerevisiae）中，已经从适合度和特定形态特征的角度深入研究了基因表型效应的大小，但对整体形态异常知之甚少。
我们鉴定出 1126 个整体形态效应因子，当它们缺失时会导致严重的整体形态异常，还有 2241 个具有弱整体效应但对酵母形态有独特影响的特定形态效应因子。整体效应因子属于许多基因功能类别，作为网络枢纽发挥作用，影响大量形态特征，与大量基因相互作用，并促进高蛋白质表达。整体形态异常可用于估计基因对形态的重要性。基因重要性对适合度和形态的贡献可用于将基因高效分类为功能组。
整体形态异常可用作高维形态表型的可重复且可靠的基因特征。它可以在许多功能基因组学应用中使用。
