Pal Mohinder, Kumari Munesh, Kiran Shashi, Salwan Richa, Mayilraj Shanmugam, Chhibber Sanjay, Gulati Arvind
CSIR-Institute of Himalayan Bioresource Technology, Palampur (HP)-176 061, India.
Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Chandigarh 160 036, India.
Int J Syst Evol Microbiol. 2018 Mar;68(3):865-870. doi: 10.1099/ijsem.0.002600. Epub 2018 Jan 29.
A novel bacterial strain, IHBB 10212, of the genus Chryseobacterium was isolated from a glacier near the Kunzum Pass located in the Lahaul-Spiti in the North-Western Himalayas of India. The cells were Gram-negative, aerobic, non-sporulating, single rods, lacked flagella, and formed yellow to orange pigmented colonies. The strain utilized maltose, trehalose, sucrose, gentibiose, glucose, mannose, fructose, mannitol, arabitol and salicin for growth. Flexirubin-type pigments were produced by strain IHBB 10212. The 16S rRNA gene sequence analysis showed relatedness of strain IHBB 10212 to Chryseobacterium polytrichastri DSM 26899 (97.43 %), Chryseobacterium greenlandense CIP 110007 (97.29 %) and Chryseobacterium aquaticum KCTC 12483 (96.80 %). Iso-C15 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) constituted the major cellular fatty acids. The polar lipids present were six unidentified aminolipids, one unidentified phospholipid and three unidentified lipids. MK-6 was identified as the major quinone. The DNA G+C content was 34.08 mol%. Digital DNA-DNA hybridization of strain IHBB 10212 with C. polytrichastri, C. greenlandense and C. aquaticum showed values far below the prescribed thresholds of 95 % for average nucleotide identity and 70 % for the Genome-to-Genome Distance Calculator for species delineation. Based on its differences from validly published Chryseobacterium species, strain IHBB 10212 is identified as a new species, for which the proposed name is Chryseobacterium glaciei sp. nov., with IHBB 10212 as the type strain (=MTCC 12457=JCM 31156=KACC 19170).
从印度喜马拉雅西北部拉胡尔-斯皮提地区昆祖姆山口附近的一座冰川中分离出一株新型的金黄杆菌属细菌菌株，即IHBB 10212。该菌细胞革兰氏阴性、需氧、不产芽孢、呈单个杆状、无鞭毛，形成黄色至橙色色素菌落。该菌株利用麦芽糖、海藻糖、蔗糖、龙胆二糖、葡萄糖、甘露糖、果糖、甘露醇、阿拉伯糖醇和水杨苷进行生长。菌株IHBB 10212产生flexirubin型色素。16S rRNA基因序列分析表明，菌株IHBB 10212与多毛金黄杆菌DSM 26899（97.43%）、格陵兰金黄杆菌CIP 110007（97.29%）和水生金黄杆菌KCTC 12483（96.80%）具有相关性。异-C15 : 0和总和特征3（C16 : 1ω7c/C16 : 1ω6c）构成主要细胞脂肪酸。存在的极性脂质为六种未鉴定的氨基脂质、一种未鉴定的磷脂和三种未鉴定的脂质。MK-6被鉴定为主要醌。DNA G+C含量为34.08 mol%。菌株IHBB 10212与多毛金黄杆菌、格陵兰金黄杆菌和水生金黄杆菌的数字DNA-DNA杂交显示，其平均核苷酸同一性值远低于规定的95%阈值，基因组到基因组距离计算器用于物种划分的值远低于70%阈值。基于其与已有效发表的金黄杆菌属物种的差异，菌株IHBB 10212被鉴定为一个新物种，提议的名称为冰川金黄杆菌（Chryseobacterium glaciei sp. nov.），以IHBB 10212作为模式菌株（=MTCC 12457=JCM 31156=KACC 19170）。
