Chen Xin Yao, Zhao Ran, Chen Zhi Ling, Liu Lei, Li Xue Dong, Li Yan Hong
College of Life Science, Capital Normal University, Xisanhuan North Road No. 105, Haidian District, Beijing, 100048, China.
Antonie Van Leeuwenhoek. 2015 Feb;107(2):403-10. doi: 10.1007/s10482-014-0338-6. Epub 2014 Dec 2.
A Gram-stain negative, rod-shaped and non-endospore forming bacterium, designated strain YG4-6(T), was isolated from Polytrichastrum formosum collected from Gawalong glacier in Tibet, China and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain YG4-6(T) were identified as iso-C15:0 (29.3 %), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c as defined by MIDI, 23.5 %) and iso-C17:0 3-OH (16.5 %). The major polar lipids were found to consist of five unidentified aminolipids and three unidentified lipids. Strain YG4-6(T) was found to contain MK-6 as the dominant menaquinone and the G+C content of its genomic DNA was determined to be 37.3 mol%. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YG4-6(T) is affiliated to Chryseobacterium species, and its closest related species were Chryseobacterium aahli T68(T) (97.9 % sequence similarity), Chryseobacterium zeae JM-1085(T) (97.8 % sequence similarity), Chryseobacterium yeoncheonense DCY67(T) (97.6 % sequence similarity) and Chryseobacterium soldanellicola NBRC 100864(T) (97.2 % sequence similarity). However, the DNA-DNA relatedness values between these strains and strain YG4-6(T) were found to be clearly below 70 %. Based on the phylogenetic inference and phenotypic data, strain YG4-6(T) is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium polytrichastri sp. nov. is proposed. The type strain is YG4-6(T) (=CGMCC 1.12491(T) = DSM 26899(T)). An emended description of the genus Chryseobacterium is also proposed.
从中国西藏嘎瓦龙冰川采集的台湾拟金发藓中分离出一株革兰氏染色阴性、杆状且不形成芽孢的细菌，命名为菌株YG4-6(T)，并采用多相分类方法对其进行了表征。菌株YG4-6(T)的主要脂肪酸被鉴定为异-C15:0（29.3%）、特征性总和3（按照MIDI定义的C16:1 ω7c和/或C16:1 ω6c，23.5%）和异-C17:0 3-OH（16.5%）。发现主要极性脂质由五种未鉴定的氨基脂质和三种未鉴定的脂质组成。菌株YG4-6(T)被发现含有MK-6作为主要甲基萘醌，其基因组DNA的G+C含量测定为37.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YG4-6(T)隶属于金黄杆菌属，与其亲缘关系最近的物种是阿氏金黄杆菌T68(T)（序列相似性97.9%）、玉米金黄杆菌JM-1085(T)（序列相似性97.8%）、涟川金黄杆菌DCY67(T)（序列相似性97.6%）和滨螺金黄杆菌NBRC 100864(T)（序列相似性97.2%）。然而，发现这些菌株与菌株YG4-6(T)之间的DNA-DNA相关性值明显低于70%。基于系统发育推断和表型数据，菌株YG4-6(T)被认为代表金黄杆菌属的一个新物种，为此提出金黄杆菌台湾拟金发藓新种（Chryseobacterium polytrichastri sp. nov.）。模式菌株为YG4-6(T)（=CGMCC 1.12491(T) = DSM 26899(T)）。还提出了金黄杆菌属的修订描述。
