Cui Meng-Di, Wang Xiang, Jiang Wan-Kui, Hu Gang, Yang Zhan-Gong, Sun Gao-Jie, Zhu Shi-Jun, Zhou Yi-Dong, Hong Qing
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
College of Resource and Environment, Anhui Science and Technology University, Anhui 233100, PR China.
Int J Syst Evol Microbiol. 2018 Mar;68(3):886-891. doi: 10.1099/ijsem.0.002604. Epub 2018 Feb 1.
A Gram-stain-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterium, designated YHM-9, was isolated from soil in Yangquan, Shanxi Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YHM-9 belonged to the genus Pedobacter and shared the highest similarity (97.4 %) to the type strain Pedobacter lignilitoris W-WS13. Strain YHM-9 exhibited low DNA-DNA relatedness with P. lignilitoris W-WS13 (21.7±1.3 %). The DNA G+C content was 38.9 mol%. The major fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH. The respiratory quinone was MK-7, the major polyamine was sym-homospermidine and the major polar lipids were phosphatidylethanolamine. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics, strain YHM-9 was recognized as a representative of a novel species within the genus Pedobacter, for which the name Pedobacteragrisoli sp. nov. is proposed. The type strain is YHM-9 (=JCM 32093=CCTCC AB 2017125).
从中国山西省阳泉市土壤中分离出一株革兰氏阴性、需氧、不运动、不产芽孢的杆状细菌，命名为YHM-9。基于16S rRNA基因序列的系统发育分析表明，菌株YHM-9属于Pedobacter属，与模式菌株Pedobacter lignilitoris W-WS13的相似度最高（97.4%）。菌株YHM-9与P. lignilitoris W-WS13的DNA-DNA相关性较低（21.7±1.3%）。DNA G+C含量为38.9 mol%。主要脂肪酸为异C15:0、特征性总和3（C16:1ω7c和/或C16:1ω6c）和异C17:0 3-OH。呼吸醌为MK-7，主要多胺为对称高亚精胺，主要极性脂为磷脂酰乙醇胺。基于形态、生理、生化及化学分类学特征，菌株YHM-9被认定为Pedobacter属内一个新物种的代表，为此提出新物种名称Pedobacteragrisoli sp. nov.。模式菌株为YHM-9（=JCM 32093=CCTCC AB 2017125）。
