Zhou Naizhen, Hu Ke, Guo Zhaobin, Zhang Qiying, Chen Junsong, Zhang Tianzhu, Gu Ning
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210096, China.
Key Lab of Clinical and Medical Engineering, Department of Biomedical Engineering, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, China.
J Nanosci Nanotechnol. 2018 Aug 1;18(8):5252-5255. doi: 10.1166/jnn.2018.15372.
Thermo-sensitive hydrogels which could encapsulate cells and provide a three dimensional (3D) microenvironment have great potential in building new cell culture models in vitro. In this study, a thermal responsive hydrogel based on PLGA-PEG-PLGA tri-block copolymers was developed as matrix for 3D ovarian cancer cell culturing. The gelation of PLGA-PEG-PLGA tri-block copolymer was concentration-dependent. SEM images showed the pores were suitable for the formation of 3D cell structures. Cell morphological results showed that large aggregates of ovarian cancer cells (HO8910) were formed after cultured for 10 days. Therefore, hydrogel based on PLGA-PEG-PLGA tri-block copolymers hold potential as in vitro cell culture matrix for ovarian cancer cells.
能够封装细胞并提供三维（3D）微环境的热敏水凝胶在构建体外新型细胞培养模型方面具有巨大潜力。在本研究中，开发了一种基于聚乳酸-聚乙二醇-聚乳酸（PLGA-PEG-PLGA）三嵌段共聚物的热响应水凝胶作为三维卵巢癌细胞培养的基质。PLGA-PEG-PLGA三嵌段共聚物的凝胶化具有浓度依赖性。扫描电子显微镜（SEM）图像显示这些孔隙适合三维细胞结构的形成。细胞形态学结果表明，卵巢癌细胞（HO8910）培养10天后形成了大的聚集体。因此，基于PLGA-PEG-PLGA三嵌段共聚物的水凝胶有望作为卵巢癌细胞的体外细胞培养基质。
