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阳离子聚合物修饰的 PLGA 纳米粒包载驴乳多糖作为卵清蛋白的疫苗传递系统以改善免疫应答。

Cationic polymer modified PLGA nanoparticles encapsulating Alhagi honey polysaccharides as a vaccine delivery system for ovalbumin to improve immune responses.

机构信息

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.

出版信息

Int J Nanomedicine. 2019 May 6;14:3221-3234. doi: 10.2147/IJN.S203072. eCollection 2019.

DOI:10.2147/IJN.S203072
PMID:31123399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6510392/
Abstract

Poly (lactic-co-glycolic acid) (PLGA) nanoparticles and surface modified PLGA nanoparticles have been widely studied as antigens or drugs carriers due to their controlled release characteristics and biocompatibility. However, most PLGA nanoparticles have lower antigens loading efficiency and adjuvanticity. The aim of this study was to improve the antigen loading efficiency and adjuvant activity of PLGA nanoparticles. Surface cationic polymer modification can improve the antigens loading efficiency of PLGA nanoparticles by surface adsorption. Therefore, in this study, chitosan modified PLGA nanoparticles (CS-AHPP/OVA), polyethyleneimine modified PLGA nanoparticles (PEI-AHPP/OVA), and ε-Poly-L-lysine modified PLGA nanoparticles (εPL-AHPP/OVA) were prepared as antigen delivery carriers to investigate the characterization and stability of these nanoparticles. These nanoparticles were evaluated for their efficacies as adjuvants pre- and post-modification. The AHP and OVA-loaded PLGA nanoparticles (AHPP/OVA) were positively charged after surface cationic polymers modification, and their structural integrity was maintained. Their antigen loading capacity and stability of nanoparticles were improved by the surface cationic polymers modification. Increased positive surface charge resulted in greater OVA adsorption capacity. Among AHPP/OVA and the three surface cationic polymers synthesized from modified PLGA nanoparticles, PEI-AHPP/OVA showed the highest antigen loading efficiency and good stability. AHPP/OVA, CS-AHPP/OVA PEI-AHPP/OVA, and εPL-AHPP/OVA formulations significantly enhanced lymphocyte proliferation and improved the ratio of CD4+/CD8+ T cells. In addition, AHPP/OVA, PEI-AHPP/OVA and εPL-AHPP/OVA formulations induced secretion of cytokines (TNF-α, IFN-γ, IL-4, and IL-6), antibodies (IgG) and antibody subtypes (IgG1 and IgG2a) in immunized mice. These results demonstrate that these formulations generated a strong Th1-biased immune response. Among them, PEI-AHPP/OVA induced the strongest Th1-biased immune response. In conclusion, PEI-AHPP/OVA nanoparticles may be a potential antigen delivery system for the induction of strong immune responses.

摘要

聚（丙交酯-乙交酯）（PLGA）纳米粒和表面修饰的 PLGA 纳米粒由于其控制释放特性和生物相容性而被广泛研究作为抗原或药物载体。然而，大多数 PLGA 纳米粒的抗原载量效率和佐剂活性较低。本研究旨在提高 PLGA 纳米粒的抗原载量效率和佐剂活性。表面阳离子聚合物修饰可以通过表面吸附提高 PLGA 纳米粒的抗原载量效率。因此，在本研究中，制备了壳聚糖修饰的 PLGA 纳米粒（CS-AHPP/OVA）、聚乙烯亚胺修饰的 PLGA 纳米粒（PEI-AHPP/OVA）和 ε-聚赖氨酸修饰的 PLGA 纳米粒（εPL-AHPP/OVA）作为抗原递送载体，以研究这些纳米粒的特性和稳定性。这些纳米粒被评估为修饰前后作为佐剂的功效。负载 AHP 和 OVA 的 PLGA 纳米粒（AHPP/OVA）在表面阳离子聚合物修饰后带正电荷，其结构完整性得以保持。其抗原载量和纳米粒的稳定性通过表面阳离子聚合物修饰得到提高。增加的正表面电荷导致 OVA 的吸附能力增加。在负载 AHP 和 OVA 的 PLGA 纳米粒（AHPP/OVA）和三种由修饰的 PLGA 纳米粒合成的表面阳离子聚合物中，PEI-AHPP/OVA 表现出最高的抗原载量效率和良好的稳定性。AHPP/OVA、CS-AHPP/OVA、PEI-AHPP/OVA 和 εPL-AHPP/OVA 制剂显著增强了淋巴细胞增殖，并提高了 CD4+/CD8+T 细胞的比值。此外，AHPP/OVA、PEI-AHPP/OVA 和 εPL-AHPP/OVA 制剂诱导免疫小鼠分泌细胞因子（TNF-α、IFN-γ、IL-4 和 IL-6）、抗体（IgG）和抗体亚型（IgG1 和 IgG2a）。这些结果表明，这些制剂产生了强烈的 Th1 偏向性免疫反应。其中，PEI-AHPP/OVA 诱导了最强的 Th1 偏向性免疫反应。综上所述，PEI-AHPP/OVA 纳米粒可能是一种潜在的抗原递送系统，可诱导强烈的免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/88af2bb3abca/IJN-14-3221-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/e7efb2f98094/IJN-14-3221-g0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/3354c4ba6600/IJN-14-3221-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/88af2bb3abca/IJN-14-3221-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/e7efb2f98094/IJN-14-3221-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/44e782f30acb/IJN-14-3221-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/913d71eaefa2/IJN-14-3221-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/e90207bc1dcb/IJN-14-3221-g0004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07b6/6510392/88af2bb3abca/IJN-14-3221-g0006.jpg

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2
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Exp Ther Med. 2018 Apr;15(4):3556-3562. doi: 10.3892/etm.2018.5818. Epub 2018 Jan 31.
3
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5
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6
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7
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J Mater Sci Mater Med. 2021 Jun 7;32(6):63. doi: 10.1007/s10856-021-06533-7.
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J Nanosci Nanotechnol. 2018 Aug 1;18(8):5252-5255. doi: 10.1166/jnn.2018.15372.
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6
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Biotechnol J. 2018 Jan;13(1). doi: 10.1002/biot.201700203. Epub 2017 Oct 13.
7
Optimization of angelica sinensis polysaccharide-loaded Poly (lactic-co-glycolicacid) nanoparticles by RSM and its immunological activity in vitro.响应面法优化当归多糖载药聚乳酸-羟基乙酸共聚物纳米粒及其体外免疫活性。
Int J Biol Macromol. 2018 Feb;107(Pt A):222-229. doi: 10.1016/j.ijbiomac.2017.08.176. Epub 2017 Sep 1.
8
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J Pharm Sci. 2017 Nov;106(11):3316-3327. doi: 10.1016/j.xphs.2017.06.013. Epub 2017 Jun 23.
10
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ACS Appl Mater Interfaces. 2017 Jul 19;9(28):23466-23475. doi: 10.1021/acsami.7b06024. Epub 2017 Jul 3.