吸附于聚乙二醇化金纳米颗粒上的转铁蛋白及其与靶向特异性的相关性。
Transferrin Adsorbed on PEGylated Gold Nanoparticles and Its Relevance to Targeting Specificity.
作者信息
Cai Chengxu, Wang Mingzhe, Wang Xinyi, Wang Bing, Chen Hanqing, Chen Jian, Chen Chunying, Feng Weiyue
机构信息
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
出版信息
J Nanosci Nanotechnol. 2018 Aug 1;18(8):5306-5313. doi: 10.1166/jnn.2018.15435.
Polyethylene glycol (PEG) has been frequently used for surface modification of nanoparticles (NPs) to reduce non-specific binding of proteins on NPs. The investigation of protein absorption on PEGylated nanoparticles is necessary. In the work, the conjugation of transferrin (Tf) to PEGylated AuNPs via adsorption or bonding was studied. The 13 nm AuNPs were coated with various molecular weight (300, 2000, 5000) carboxyl and methoxy PEG thiol. The presence of Tf on PEGylated AuNP was characterized by dynamic light scattering (DLS) and infrared spectroscopy (IR). The data of IR confirmed the presence of Tf on PEGylated AuNPs. The diameter decrease of PEGylated AuNPs after Tf adsorption was observed by DLS measurement, which is attributed to competitive adsorption between Tf and PEG molecules. These phenomena may be important to the preservation of Tf targeting specificity on PEGylated AuNPs.
聚乙二醇（PEG）已被频繁用于纳米颗粒（NPs）的表面修饰，以减少蛋白质在纳米颗粒上的非特异性结合。研究蛋白质在聚乙二醇化纳米颗粒上的吸附是必要的。在这项工作中，研究了转铁蛋白（Tf）通过吸附或键合与聚乙二醇化金纳米颗粒（AuNPs）的结合。13纳米的金纳米颗粒用各种分子量（300、2000、5000）的羧基和甲氧基聚乙二醇硫醇进行了包覆。通过动态光散射（DLS）和红外光谱（IR）对聚乙二醇化金纳米颗粒上Tf的存在进行了表征。红外光谱数据证实了聚乙二醇化金纳米颗粒上存在Tf。通过DLS测量观察到Tf吸附后聚乙二醇化金纳米颗粒的直径减小，这归因于Tf和PEG分子之间的竞争性吸附。这些现象对于聚乙二醇化金纳米颗粒上Tf靶向特异性的保留可能很重要。
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