Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.
Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.
Mar Environ Res. 2018 May;136:179-189. doi: 10.1016/j.marenvres.2018.02.012. Epub 2018 Feb 12.
Biochemical and cellular responses to low concentrations of TiO nanoparticles (nTiO 1 and 10 μg/L) and bulk (bTiO, 10 μg/L) were evaluated in gills, digestive gland and haemolymph of the clam Ruditapes philippinarum after1, 3 and 7 days' exposure. At 7 days, titanium content was determined in gills and digestive gland. nTiO significantly increased antioxidant enzyme activities in both tissues, and lipid peroxidation in digestive gland at 10 μg/L only, and affected glutathione S-transferase activity. Slighter variations were observed in bTiO-treated clams. A significant Ti bioaccumulation was detected in both gills and digestive gland of 10 μg nTiO/L-exposed clams. In haemolymph, nTiO affected total haemocyte count, haemocyte proliferation, haemocyte diameter and volume, and induced DNA damage. Overall, this study demonstrated that TiO alters most of the biomarkers measured in clams, although responses were differently modulated depending on tissues and exposure conditions, and indicated that nTiO can be accumulated by bivalves, suggesting a potential risk for filter-feeding animals.
在贻贝 Ruditapes philippinarum 暴露于低浓度 TiO 纳米颗粒（nTiO 1 和 10μg/L）和体相 TiO（bTiO，10μg/L）1、3 和 7 天后，评估了其鳃、消化腺和血淋巴中的生化和细胞反应。在第 7 天，测定了鳃和消化腺中的钛含量。nTiO 在两种组织中均显著增加了抗氧化酶活性，在 10μg/L 时仅在消化腺中增加了脂质过氧化作用，并影响了谷胱甘肽 S-转移酶活性。在 bTiO 处理的贻贝中观察到较轻的变化。在 10μg nTiO/L 暴露的贻贝的鳃和消化腺中均检测到显著的 Ti 生物积累。在血淋巴中，nTiO 影响总血细胞计数、血细胞增殖、血细胞直径和体积，并诱导 DNA 损伤。总体而言，本研究表明 TiO 改变了贻贝中大多数测量的生物标志物，尽管反应因组织和暴露条件而异，并且表明 nTiO 可以被双壳类动物积累，这表明对滤食性动物存在潜在风险。
