Science and Engineering Faculty, Queensland University of Technology, 2 George Street, Brisbane QLD 4001, Australia.
Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove QLD 4059, Australia.
J Mech Behav Biomed Mater. 2018 Apr;80:311-319. doi: 10.1016/j.jmbbm.2018.02.011. Epub 2018 Feb 12.
The application of orthopaedic implants is associated with risks of bacterial infection and long-term antibiotic therapy. This problem has led to the study of implants with nano-textured surfaces as a method of inhibiting bacterial adhesion and reducing implant failure due to infection. In this research, various nano-textured surfaces of TiO were synthesised using hydrothermal synthesis, by varying NaOH concentration, reaction time and reaction temperature. Their correlations to mechanical, morphological, bactericidal and osteogenic properties of the surfaces were investigated. It was found that high alkaline concentrations produced large nanowire mesh arrays, while short reaction time and low temperature produced comparatively smaller arrays. The highly dense morphology formed at higher NaOH concentrations has resulted in high elastic modulus and hardness values, compared to surfaces produced at lower NaOH concentrations. Viability tests of the TiO nanowire array against gram-positive Staphylococcus aureus cells showed a bactericidal efficiency of 54% and 33% after 3 and 18 h, respectively. This nano-textured surface produces an osteoblast cellular metabolic activity of 71% after 24 h, compared to 67% when exposed to a flat Ti control surface. This preliminary work demonstrates an excellent outcome in producing bactericidal surfaces that promoted metabolic activity of human osteoblast cells for potential use in orthopaedic implants.
骨科植入物的应用与细菌感染和长期抗生素治疗的风险有关。这个问题促使人们研究具有纳米纹理表面的植入物，作为抑制细菌黏附并降低因感染导致植入物失效的方法。在这项研究中，使用水热合成法通过改变 NaOH 浓度、反应时间和反应温度来合成各种 TiO 的纳米纹理表面。研究了它们与表面的机械、形态、杀菌和成骨性能的相关性。结果发现，高碱性浓度会产生大的纳米线网阵列，而短反应时间和低温会产生相对较小的阵列。与在较低 NaOH 浓度下生成的表面相比，在较高 NaOH 浓度下形成的高密度形态导致较高的弹性模量和硬度值。TiO 纳米线阵列对革兰氏阳性金黄色葡萄球菌细胞的活菌测试表明，分别在 3 和 18 小时后杀菌效率为 54%和 33%。与暴露于平 Ti 对照表面相比，这种纳米纹理表面在 24 小时后产生的成骨细胞代谢活性为 71%。这项初步工作表明，在制备杀菌表面方面取得了优异的效果，这些表面促进了人成骨细胞的代谢活性，有望用于骨科植入物。
