Department of Chemical Science, Biochemistry Unit, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
Department of Chemical Science, Biochemistry Unit, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
Biomed Pharmacother. 2018 May;101:8-13. doi: 10.1016/j.biopha.2018.01.156. Epub 2018 Feb 16.
Bryophyllum pinnatum (B. pinnatum) (Lam.) Oken is used in tropical Africa for the treatment of several diseases such as kidney and urinary disorders. This study was aimed to evaluate the effect of B. pinnatum on arginase II activity and its prevention against renal oxidative damage occasioned by CCl in rats. Rats were randomly divided into six groups; group I served as the control, group II served as carbon tetrachloride (CCl) intoxicated group, group III-V animals were pre-treated with silymarin (25 mg/kg body weight), 25 mg/kg body weight aqueous extracts of Bryophyllum pinnatum (AEBP) and 50 mg/kg body weight AEBP, respectively, for 14 days, followed by a single injection of CCl Group VI rats received AEBP only (50 mg/kg body weight). Results obtained revealed that CCl intoxication significantly increased (p < 0.05) the levels of renal markers (serum urea, creatinine and arginase II) in rats when compared to the control group. Further, oxidative stress status appeared in CCl-intoxicated rats, as evidence by significant elevation in malondialdehyde (MDA), with concomitant decrease in levels of functional sulfhydryl groups (SH), antioxidant enzymes and nitric oxide in rats' kidney. These adverse changes, due to CCl intoxication in rats, were however, prevented by pre-treatment with AEBP leaves (25 and 50 mg/kg body weight). The inhibition of arginase II, as well as increased antioxidant status by AEBP in CCl-intoxicated rats suggests that B. pinnatum can protect kidney against CCl-induced oxidative damage.
落地生根（B. pinnatum）（Lam.）Oken 被用于治疗热带非洲的多种疾病，如肾脏和泌尿系统疾病。本研究旨在评估落地生根对精氨酸酶 II 活性的影响及其对 CCl 引起的大鼠肾氧化损伤的预防作用。大鼠随机分为六组；第 I 组为对照组，第 II 组为四氯化碳（CCl）中毒组，第 III-V 组动物分别用水飞蓟素（25mg/kg 体重）、25mg/kg 体重落地生根水提物（AEBP）和 50mg/kg 体重 AEBP 预处理 14 天，然后单次注射 CCl 组 VI 大鼠仅接受 AEBP（50mg/kg 体重）。结果表明，与对照组相比，CCl 中毒显著增加了大鼠肾脏标志物（血清尿素、肌酐和精氨酸酶 II）的水平（p<0.05）。此外，CCl 中毒大鼠的氧化应激状态表现为丙二醛（MDA）显著升高，同时大鼠肾脏中的功能巯基（SH）、抗氧化酶和一氧化氮水平降低。这些由于 CCl 中毒导致的不利变化，然而，用 AEBP 叶片（25 和 50mg/kg 体重）预处理可以预防。AEBP 在 CCl 中毒大鼠中抑制精氨酸酶 II 并增加抗氧化状态表明，落地生根可以保护肾脏免受 CCl 引起的氧化损伤。
