Anadozie Scholastica Onyebuchi, Akinyemi Jacob Ayodele, Adewale Olusola Bolaji, Isitua Christy Chinwe
Department of Chemical Sciences, Biochemistry unit, Afe Babalola University, Km 8.5 Afe Babalola way, PMB 5454, Ado-Ekiti, Ekiti State, Nigeria, Phone: +234 813 3254 737; +27 64 63 180 64.
Department of Chemical Sciences, Biochemistry unit, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
J Basic Clin Physiol Pharmacol. 2019 Sep 9;30(5):jbcpp-2018-0161. doi: 10.1515/jbcpp-2018-0161.
Background Short-term memory impairment is a neurodegenerative disease associated with oxidative stress. Bryophyllum pinnatum (Lam.) Oken of the family Crassulaceae is traditionally used in the treatment of diseases, such as cough, wounds, and kidney diseases. This study evaluates the effect of the aqueous extract of B. pinnatum (AEBP) leaves on acetylcholinesterase activity in carbon tetrachloride (CCl4)-induced short-term memory impairment in rats. Methods Thirty male Wistar albino rats were used in this study and were divided into six groups (n=5). Group I served as control, group II rats were induced with CCl4, while groups III-V animals were pretreated with silymarin (25 mg/kg body weight), 25 and 50 mg/kg body weight AEBP leaves, respectively, once daily by oral gavage for 14 days prior to a single intraperitoneal injection of CCl4. Animals in group VI received 50 mg/kg body weight AEBP only by oral gavage. Results Administration of carbon tetrachloride significantly increased (p<0.05) spontaneous alternation and locomotor function in rats when compared with the control group. Also, the levels of acetylcholinesterase, adenosine deaminase, and malondialdehyde were increased in CCl4-administered rats, with reduction in both enzymatic and nonenzymatic antioxidant levels. However, pretreatment of rats with AEBP leaves, at tested doses, prevented these changes. Conclusions The increased antioxidant status and the inhibition of acetylcholinesterase activity show that AEBP leaves improve learning memory and stabilizes memory impairment caused by CCl4.
背景 短期记忆障碍是一种与氧化应激相关的神经退行性疾病。景天科落地生根属的落地生根传统上用于治疗咳嗽、伤口和肾脏疾病等病症。本研究评估了落地生根叶水提取物（AEBP）对四氯化碳（CCl4）诱导的大鼠短期记忆障碍中乙酰胆碱酯酶活性的影响。方法 本研究使用30只雄性Wistar白化大鼠，分为六组（n = 5）。第一组作为对照组，第二组大鼠用CCl4诱导，而第三至五组动物在单次腹腔注射CCl4前14天，分别用25mg/kg体重的水飞蓟宾、25mg/kg体重和50mg/kg体重的落地生根叶进行预处理，每天经口灌胃一次。第六组动物仅经口灌胃给予50mg/kg体重的AEBP。结果 与对照组相比，给予四氯化碳显著增加（p<0.05）了大鼠的自发交替和运动功能。此外，给予CCl4的大鼠中乙酰胆碱酯酶、腺苷脱氨酶和丙二醛水平升高，而酶促和非酶促抗氧化剂水平降低。然而，用测试剂量的落地生根叶对大鼠进行预处理可防止这些变化。结论 抗氧化状态的提高和乙酰胆碱酯酶活性的抑制表明，AEBP叶可改善学习记忆并稳定由CCl4引起的记忆障碍。
