Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
J Environ Manage. 2018 Apr 15;212:375-383. doi: 10.1016/j.jenvman.2018.02.032. Epub 2018 Feb 22.
Composting of cattle manure was conducted under four ventilation strategies, i.e., no-aeration (A-00), continuous aeration (B-44), non-aeration for 14 d and then aeration for 42 d (C-04), aeration for 14 d and then no-aeration for 42 d (D-40). Physicochemical parameters and potential ammonia oxidation (PAO) indicated that continuous and intermittent ventilation provide favourable conditions for ammonia-oxidizing bacteria (AOB) and archaea (AOA) to oxidize ammonia. Quantitative PCR (qPCR) analysis showed AOB amoA gene abundance of all treatments on every sampling day ranged from 2.25 × 10 to 2.76 × 10copies/g, was significantly lower than that of archaeal amoA gene from 2.71 × 10 to 9.05 × 10copies/g. There was also a significantly positive relationship between PAO rates and AOB (r ≥ 0.066, p < 0.05) and AOA (r ≥ 0.300, p < 0.05) abundance. These data suggested that ammonia oxidation is driven by both AOA and AOB in cattle manure composting.
牛粪堆肥在四种通风策略下进行，分别为不曝气（A-00）、连续曝气（B-44）、先不曝气 14 天然后曝气 42 天（C-04）和先曝气 14 天然后不曝气 42 天（D-40）。理化参数和潜在氨氧化（PAO）表明，连续和间歇通风为氨氧化细菌（AOB）和古菌（AOA）氧化氨提供了有利条件。定量 PCR（qPCR）分析显示，所有处理组在每个采样日的 AOB amoA 基因丰度范围为 2.25×10 到 2.76×10 拷贝/g，明显低于古菌 amoA 基因的丰度范围 2.71×10 到 9.05×10 拷贝/g。PAO 速率与 AOB（r≥0.066，p<0.05）和 AOA（r≥0.300，p<0.05）丰度之间也存在显著的正相关关系。这些数据表明，氨氧化是由牛粪堆肥中的 AOA 和 AOB 共同驱动的。
