Beppu Fumiaki, Kondo Hitomi, Kasatani Satoshi, Aoki Yoshinori, Gotoh Naohiro
Department of Food Science and Technology, Tokyo University of Marine Science and Technology.
Laboratory Section Division III, MITSUBISHI-CHEMICAL FOODS CORPORATION.
J Oleo Sci. 2018 Mar 1;67(3):283-288. doi: 10.5650/jos.ess17218. Epub 2018 Feb 19.
In this study, we measured the quantity of marine-derived tocopherol (MDT), a monounsaturated vitamin E (VE), stored in the body tissue of mice fed with a diet containing a VE-rich fraction extracted from salmon roe. We first prepared the calibration curves for the MDT concentration using an HPLC-fluorescence system. Ranging from 0.016 to 50 μg/mL, the slope was expressed as first-order equations, with R values = 0.99. The mice were fed with an AIN-93 based diet containing MDT in doses of 21.4 mg/kg for 4 weeks, and the storage in the heart, lung, liver, stomach, small intestine, large intestine, kidney, pancreas, spleen, testis, skeletal muscle, visceral white adipose tissue (WAT), subcutaneous WAT and brain was quantified. MDT was widely distributed in tissues throughout the whole body, with higher accumulations observed in the adipose tissue, liver and kidney. These results demonstrate means to estimating the MDT concentration in natural products and in the bodies of animals and contribute to the understanding of the physiological functions of MDT in relation to human health.
在本研究中，我们测量了海洋来源生育酚（MDT）的含量，MDT是一种单不饱和维生素E（VE），储存在喂食含有从鲑鱼籽中提取的富含VE组分的饮食的小鼠的身体组织中。我们首先使用高效液相色谱-荧光系统制备了MDT浓度的校准曲线。浓度范围为0.016至50μg/mL，斜率表示为一阶方程，R值=0.99。给小鼠喂食基于AIN-93的饮食，其中MDT剂量为21.4mg/kg，持续4周，并对心脏、肺、肝脏、胃、小肠、大肠、肾脏、胰腺、脾脏、睾丸、骨骼肌、内脏白色脂肪组织（WAT）、皮下WAT和大脑中的储存量进行了定量。MDT广泛分布于全身组织中，在脂肪组织、肝脏和肾脏中积累较多。这些结果证明了估计天然产物和动物体内MDT浓度的方法，并有助于理解MDT与人类健康相关的生理功能。
