Department of Nutrition and Life Science, Kanagawa Institute of Technology.
Department of Pharmacology, National Center for Child Health and Development.
J Oleo Sci. 2021 Feb 1;70(2):247-252. doi: 10.5650/jos.ess20254. Epub 2021 Jan 15.
This study aimed to compare the distribution of vitamin E analogs, particularly α-tocopherol and δ-tocopherol, in mice fed with a normal diet and a high-fat and high-sucrose diet separately. We used male C57BL/6JJcl strain mice, which were divided into six groups (control [C], Cα, Cδ, high-fat and high-sucrose [H], Hα, and Hδ groups) and bred for 4 weeks. The additional quantity of α-tocopherol or E-mix D (containing 86.7% δ-tocopherol) into diet was 800 mg/kg diet. The final body weight was significantly higher in the H group than in the C group. However, the effects of vitamin E analog intake had no significant difference, with no synergy between vitamin E and diet. Similar results were obtained in epididymal fat weight. Moreover, α-tocopherol was mainly distributed in the liver in both the Cα group and Hα group, whereas δ-tocopherol mostly accumulated in the epididymal fat, in both the Cδ group and Hδ group. Also, δ-tocopherol was detected in all tissues in both groups. Both the α-tocopherol and δ-tocopherol levels in the epididymal fat were significantly lower in the H group than in the C group. In conclusion, our results suggest that a portion of δ-tocopherol was incorporated into the adipose tissue by chylomicron before arriving at the liver, and then it is metabolized in the liver.
本研究旨在比较正常饮食和高脂肪高蔗糖饮食喂养的小鼠中维生素 E 类似物（特别是α-生育酚和δ-生育酚）的分布。我们使用雄性 C57BL/6JJcl 品系小鼠，将其分为六组（对照组[C]、Cα、Cδ、高脂肪高蔗糖组[H]、Hα 和 Hδ 组），并繁殖 4 周。饮食中额外添加的α-生育酚或 E-混合 D（含有 86.7%δ-生育酚）的量为 800mg/kg 饮食。H 组的最终体重明显高于 C 组。然而，维生素 E 类似物摄入的影响没有显著差异，维生素 E 与饮食之间没有协同作用。附睾脂肪重量也得到了类似的结果。此外，α-生育酚主要分布在 Cα 组和 Hα 组的肝脏中，而 δ-生育酚主要积聚在 Cδ 组和 Hδ 组的附睾脂肪中。此外，两组均检测到 δ-生育酚存在于所有组织中。H 组附睾脂肪中的α-生育酚和 δ-生育酚水平均明显低于 C 组。综上所述，我们的结果表明，一部分 δ-生育酚在到达肝脏之前通过乳糜微粒被纳入脂肪组织，然后在肝脏中被代谢。
