叶黄素膳食补充剂中的天然生育酚会影响幼鼠体内生育酚的组织分布。

Natural tocopherols in a dietary supplement of lutein affect tissue distribution of tocopherols in young rats.

作者信息

Jenkins M Y, Mitchell G V, Grundel E

机构信息

Office of Nutritional Products, Labeling, and Dietary Supplements, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, MD 20708, USA.

出版信息

Nutr Cancer. 2000;37(2):207-14. doi: 10.1207/S15327914NC372_14.

DOI:10.1207/S15327914NC372_14

PMID:11142095

Abstract

The effects of graded levels of Flora GLO (FG), a dietary supplement containing lutein derived from marigold flowers, on tissue concentrations of lutein and alpha- and gamma-tocopherol were determined. Six groups of male weanling Fischer 344 rats (15/group) were fed ad libitum modified AIN-93G diets containing 0%, 0.30%, 0.60%, 1.20%, 2.40%, or 4.80% FG for eight weeks. FG provided 0%, 0.015%, 0.030%, 0.060%, 0.120%, or 0.240% lutein; corresponding levels of tocopherols were approximately 0%, 0.006%, 0.012%, 0.023%, 0.046%, and 0.092%, respectively. Maximal uptakes of lutein in plasma, spleen, and liver were observed with 4.8% FG. Lutein was not detectable in brain, heart, lung, testes, and kidney. Concentrations of alpha-tocopherol in the plasma (microgram/mg triglycerides) were reduced in all rats fed FG (p < 0.05). alpha-Tocopherol increased nonlinearly in lung, heart, and spleen with increased FG level. alpha-Tocopherol in liver increased with increased dietary FG; alpha-tocopherol in kidney, testes, and brain did not increase. At each dietary level of FG, the spleen had a higher percentage of gamma-tocopherol, and testes and brain had a lower percentage of gamma-tocopherol than all other tissues. For all tissues, the concentrations of alpha-tocopherol were always higher than those of gamma-tocopherol. Tocopherols added to the dietary supplement for functional purposes appeared to have a significant effect on tissue levels of tocopherols. Therefore, changes in alpha- and gamma-tocopherol levels in tissues of rats fed FG cannot be attributed solely to effects of lutein.

摘要

本研究测定了不同剂量的弗洛拉GLO（FG）（一种含有从万寿菊中提取的叶黄素的膳食补充剂）对叶黄素以及α-生育酚和γ-生育酚组织浓度的影响。将六组雄性断乳费希尔344大鼠（每组15只）自由采食含0%、0.30%、0.60%、1.20%、2.40%或4.80% FG的改良AIN - 93G日粮，持续8周。FG提供了0%、0.015%、0.030%、0.060%、0.120%或0.240%的叶黄素；相应的生育酚水平分别约为0%、0.006%、0.012%、0.023%、0.046%和0.092%。在日粮中添加4.8% FG时，血浆、脾脏和肝脏中叶黄素的摄取量最高。在脑、心脏、肺、睾丸和肾脏中未检测到叶黄素。所有喂食FG的大鼠血浆中α-生育酚浓度（微克/毫克甘油三酯）均降低（p < 0.05）。随着日粮中FG水平的增加，肺、心脏和脾脏中的α-生育酚呈非线性增加。肝脏中的α-生育酚随着日粮中FG的增加而增加；肾脏、睾丸和脑中的α-生育酚未增加。在每个FG日粮水平下，脾脏中γ-生育酚的百分比高于其他所有组织，而睾丸和脑中γ-生育酚的百分比低于其他所有组织。对于所有组织，α-生育酚的浓度始终高于γ-生育酚。为实现功能目的添加到膳食补充剂中的生育酚似乎对组织中生育酚的水平有显著影响。因此，喂食FG的大鼠组织中α-生育酚和γ-生育酚水平的变化不能仅归因于叶黄素的作用。