Al-Okbi Sahar Y, Mohamed Doha A, Hamed Thanaa E, Edris Amr E, Fouda Karem
Nutrition and Food Sciences Department, National Research Centre.
Aroma and Flavor Chemistry Department, National Research Centre.
J Oleo Sci. 2018 Mar 1;67(3):345-353. doi: 10.5650/jos.ess17204. Epub 2018 Feb 19.
The aim of the present research was to investigate the effect of fish oil, crude Nigella sative oil and combined fish oil/Nigella sative volatile oil as hepato-regenerative and renal protective supplements. The oils were administered as emulsions to rat model with liver injury induced by CCl. Plasma activities of transaminases (AST and ALT) were evaluated as liver function indicators, while plasma creatinine and urea and creatinine clearance were determined as markers of kidney function. Plasma malondialdehyde (MDA), nitrite (NO) and tumor necrosis factor-α (TNF-α) were estimated to assess the exposure to oxidative stress and subsequent inflammation. Liver fat was extracted and their fatty acids´ methyl esters were determined using gas chromatography. Results showed that plasma activities of AST and ALT were significantly higher in CCl control group compared to control healthy group. Plasma levels of creatinine and urea increased significantly in CCl control, while creatinine clearance was reduced significantly in the same group. All rat treated groups given the three oil emulsions showed improvement in liver function pointing to the initiation of liver regeneration. The combination of fish oil/Nigella sative volatiles showed the most promising regenerative activity. Oxidative stress and inflammation which were increased significantly in CCl control group showed improvement on administration of the three different oil emulsions. Fatty acids methyl ester of liver fat revealed that rats treated with fish oil/Nigella sative volatile oil presented the highest content of unsaturated fatty acids (45.52% ± 0.81) while fish oil showed the highest saturated fatty acids (53.28% ± 1.68). Conclusion; Oral administration of oil emulsions of native fish oil, Nigella sative crude oil and combined fish oil/Nigella sative volatile oil reduced liver and kidney injury in rat model of CCl through exerting anti-inflammatory and antioxidant activity. Fish oil/Nigella sative volatile oil emulsion was the most promising hepato-regenerative and reno-protective formula among the different groups.
本研究的目的是调查鱼油、黑种草籽油以及鱼油/黑种草籽挥发油组合作为肝脏再生和肾脏保护补充剂的效果。将这些油制成乳剂，给予由四氯化碳诱导肝损伤的大鼠模型。评估转氨酶（AST和ALT）的血浆活性作为肝功能指标，同时测定血浆肌酐、尿素和肌酐清除率作为肾功能指标。估计血浆丙二醛（MDA）、亚硝酸盐（NO）和肿瘤坏死因子-α（TNF-α）以评估氧化应激暴露及随后的炎症反应。提取肝脏脂肪，并用气相色谱法测定其脂肪酸甲酯。结果显示，与健康对照组相比，四氯化碳对照组的AST和ALT血浆活性显著更高。四氯化碳对照组的血浆肌酐和尿素水平显著升高，而该组的肌酐清除率显著降低。所有给予三种油乳剂的大鼠治疗组肝功能均有改善，表明肝脏再生开始。鱼油/黑种草籽挥发油组合显示出最有前景的再生活性。四氯化碳对照组中显著增加的氧化应激和炎症在给予三种不同油乳剂后有所改善。肝脏脂肪的脂肪酸甲酯显示，用鱼油/黑种草籽挥发油治疗的大鼠不饱和脂肪酸含量最高（45.52%±0.81），而鱼油的饱和脂肪酸含量最高（53.28%±1.68）。结论：口服天然鱼油、黑种草籽油以及鱼油/黑种草籽挥发油乳剂可通过发挥抗炎和抗氧化活性减轻四氯化碳诱导的大鼠模型的肝肾损伤。在不同组中，鱼油/黑种草籽挥发油乳剂是最有前景的肝脏再生和肾脏保护配方。
