Zor Murat, Aslan Elçin Latife
Lokman Hekim University, Department of Pharmacognosy, Ankara, Turkey.
Lokman Hekim University, Department of Medical Biology, Ankara, Turkey.
Turk J Pharm Sci. 2020 Feb;17(1):115-118. doi: 10.4274/tjps.galenos.2020.09471. Epub 2020 Feb 19.
Cyclophosphamide (CP) is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of CP has been studied in a variety of and systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine (), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of oil at different concentrations against the genotoxic effects of CP by micronucleus test.
For this purpose, healthy cells were treated with oil at concentrations of 1, 5, 10 μg/mL and CP as positive control for 68 hours. The micronuclei were then counted.
No significant increase in micronucleus frequency was observed when the application of oil at concentrations of 1, 5, 10 μg/mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 μg/mL) compared to the CP group in the groups treated with a oil and CP.
It has been shown that oil may have protective effects against genotoxicity agents . But more work is needed to understand the mechanism of the genotoxicity effects of oil.
环磷酰胺（CP）是一种烷化剂，广泛用作抗肿瘤和免疫抑制剂。CP的遗传毒性已在多种细胞和系统中进行了研究，并且在遗传毒性试验中常规用作阳性对照。毛茛科植物黑种草（），尤其在地中海东部国家，自古以来在许多国家被广泛用作香料和民间药物，自狄奥斯科里季斯时代起就被用作一种植物。在本研究中，旨在通过微核试验显示不同浓度的黑种草油对CP遗传毒性作用的保护作用。
为此，将健康细胞分别用浓度为1、5、10μg/mL的黑种草油处理，并以CP作为阳性对照处理68小时。然后对微核进行计数。
与阴性对照相比，当应用浓度为1、5、10μg/mL的黑种草油时，未观察到微核频率有显著增加。在用黑种草油和CP处理的组中，与CP组相比，所有三种浓度（1、5、10μg/mL）的微核数量均有所减少。
已表明黑种草油可能对遗传毒性剂具有保护作用。但需要更多的研究来了解黑种草油遗传毒性作用的机制。
