Assessment of Antigenotoxic Effect of Oil.

OBJECTIVES

Cyclophosphamide (CP) is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of CP has been studied in a variety of and systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine (), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of oil at different concentrations against the genotoxic effects of CP by micronucleus test.

MATERIALS AND METHODS

For this purpose, healthy cells were treated with oil at concentrations of 1, 5, 10 μg/mL and CP as positive control for 68 hours. The micronuclei were then counted.

RESULTS

No significant increase in micronucleus frequency was observed when the application of oil at concentrations of 1, 5, 10 μg/mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 μg/mL) compared to the CP group in the groups treated with a oil and CP.

CONCLUSION

It has been shown that oil may have protective effects against genotoxicity agents . But more work is needed to understand the mechanism of the genotoxicity effects of oil.