El-Hadary Abdalla E, Ramadan Hassanien Mohamed F
a Department of Biochemistry , Faculty of Agriculture, Banha University , Banha , Egypt and.
b Department of Agricultural Biochemistry , Faculty of Agriculture, Zagazig University , Zagazig , Egypt.
Pharm Biol. 2016 Aug;54(8):1364-72. doi: 10.3109/13880209.2015.1078381. Epub 2015 Oct 6.
Contexts: Exposure to environmental pollutants such as carbon tetrachloride (CCl4) causes liver injuries. There are claims that extracts from Syzygium aromaticum (Linn.) Merrill & L.M.Perry, (Myrtaceae) protects from such injuries.
This study investigates the protective effects of cold-pressed S. aromaticum oil (CO) against CCl4-induced liver toxicity in rats.
CO was orally administered to rats in two doses (100 and 200 mg/kg) along with CCl4 (1 mL/kg in olive oil) for 8 weeks. Indices of liver and kidney functions, lipid profile, and peroxidation were evaluated in rats' serum and tissues. Fatty acids and bioactive lipids of CO were analyzed.
High levels of monounsaturated fatty acids (39.7%) and polyunsaturated fatty acids (42.1%) were detected in CO. The oil contained high amounts of tocols and phenolics. The LD50 value at 24 h was approximately 5950 mg/kg. Treatment with 200 mg/kg CO resulted in a decrease of creatinine, urea, and uric acid levels to 0.86, 32.6, and 2.99 mg/dL, respectively. Levels of TL, TC, TAG, LDL-C, and VLDL-C were decreased to 167, 195.3, 584.5, 74.6, and 39.0 mg/L, respectively, after 8 weeks of treatment. Hepatic malondialdehyde levels were reduced and glutathione levels were elevated in CO-treated rats. CO reduced the activities of AST, ALT, and ALP as well as kidney function markers, protein, and lipid profiles, respectively. Histopathological examination of liver indicated that CO treatment reduced fatty degenerations, cytoplasmic vacuolization, and necrosis.
CO possessed a protective effect against CCl4-induced hepatotoxicity in rats, mediated possibly by the antioxidant properties of the oil.
背景：接触四氯化碳（CCl4）等环境污染物会导致肝损伤。有人声称，丁香（桃金娘科）提取物可预防此类损伤。
本研究调查冷榨丁香油（CO）对CCl4诱导的大鼠肝毒性的保护作用。
将CO以两种剂量（100和200mg/kg）口服给予大鼠，同时给予CCl4（1mL/kg溶于橄榄油），持续8周。评估大鼠血清和组织中的肝功能、肾功能、血脂谱和过氧化指标。分析了CO的脂肪酸和生物活性脂质。
在CO中检测到高水平的单不饱和脂肪酸（39.7%）和多不饱和脂肪酸（42.1%）。该油含有大量的生育酚和酚类物质。24小时的半数致死量值约为5950mg/kg。用200mg/kg CO处理后，肌酐、尿素和尿酸水平分别降至0.86、32.6和2.99mg/dL。治疗8周后，总脂、总胆固醇、甘油三酯、低密度脂蛋白胆固醇和极低密度脂蛋白胆固醇水平分别降至167、195.3、584.5、74.6和39.0mg/L。CO处理的大鼠肝丙二醛水平降低，谷胱甘肽水平升高。CO分别降低了天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶的活性以及肾功能指标、蛋白质和血脂谱。肝脏组织病理学检查表明，CO处理减少了脂肪变性、细胞质空泡化和坏死。
CO对CCl4诱导的大鼠肝毒性具有保护作用，可能是通过该油的抗氧化特性介导的。
