Jing Li, Haitao Wang, Qiong Wang, Fu Zhu, Nan Zhang, Xuezheng Zhang
Department of Rehabilitation, Weifang Hospital of Traditional Chinese Medicine, Shanghai, 261041, China.
Department of Internal Medicine, Weifang Hospital of Traditional Chinese Medicine, Shanghai, 261041, China.
Cytotechnology. 2018 Apr;70(2):855-864. doi: 10.1007/s10616-018-0198-4. Epub 2018 Feb 19.
Early diagnosis and changes associated with atherosclerosis are crucial in clinical medicine. However, atherosclerosis is a multifactorial disease. Asiaticoside (AA), a triterpenoid derived from Centella asiatica, has anti-inflammatory activity. Endothelium-derived nitric oxide is important in modulating vascular tone in a distinct vessel size-dependent manner; it plays a dominant role in conduit arteries and endothelium-dependent hyperpolarisation in resistance vessels. This study evaluated the effects of AA administration on human umbilical endothelial cells with oxidised low-density lipoprotein-induced inflammation. We measured the levels of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Our results indicated that 10-30 μM AA modulated endothelial hyper permeability, adenosine triphosphate levels, ICAM-1 expression, VCAM-1 expression, E-selectin levels, and PECAM-1 expression to 90% (p < 0.005), 80% (p < 0.05), 105% (p < 0.01), 65% (p < 0.005), 70% (p < 0.05), and 105% (p < 0.01), respectively. Taken together, our data suggest that AA inhibits the augmentation of endothelial permeability, thus preventing the early events of atherosclerosis.
早期诊断以及与动脉粥样硬化相关的变化在临床医学中至关重要。然而，动脉粥样硬化是一种多因素疾病。积雪草苷（AA）是一种从积雪草中提取的三萜类化合物，具有抗炎活性。内皮源性一氧化氮以独特的血管大小依赖性方式调节血管张力，在传导动脉中起主导作用，在阻力血管中起内皮依赖性超极化作用。本研究评估了给予AA对氧化型低密度脂蛋白诱导炎症的人脐静脉内皮细胞的影响。我们测量了细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的水平。我们的结果表明，10 - 30 μM的AA分别将内皮细胞高通透性、三磷酸腺苷水平、ICAM-1表达、VCAM-1表达、E-选择素水平和血小板内皮细胞黏附分子-1（PECAM-1）表达调节至90%（p < 0.005）、80%（p < 0.05）、105%（p < 0.01）、65%（p < 0.005）、70%（p < 0.05）和105%（p < 0.01）。综上所述，我们的数据表明AA可抑制内皮通透性的增加，从而预防动脉粥样硬化的早期事件。
