Department of Hematology, Yantai Yuhuangding Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2018 Feb;22(3):763-770. doi: 10.26355/eurrev_201802_14310.
To detect the expression of long non-coding RNA-CRNDE in patients with acute myeloid leukemia and its effect on proliferation and apoptosis in acute myeloid leukemia cell line U937.
81 cases of newly diagnosed acute myeloid leukemia (AML) were enrolled, and 35 non-malignant hematological patients were selected as controls. Quantitative RT-PCR (qRT-PCR) was performed to detect the expression of lncRNA-CRNDE in the bone marrow specimens of the subjects, and the difference between the two groups was also compared. The correlation between the expression of lncRNA-CRNDE and the sex, age, classification and total survival of clinical patients was analyzed according to the clinical data. U937 cells and monocytes isolated from normal people were cultured, and the expression of lncRNA-CRNDE in acute myeloid leukemia cell line U937 and normal monocytes was compared. SiRNA-CRNDE and pcDNA-CRNDE were transfected into U937 cells, and cell counting kit-8 (CCK-8) assay was performed to detect proliferation of U937 cells, Annexin V/PI flow cytometry was carried out to detect cell apoptosis. Cell cycle was measured by flow cytometry.
The expression of lncRNA-CRNDE in patients with AML and U937 cells was significantly higher than that in non-malignant hematological controls. Results of clinical data showed that the expression of lncRNA-CRNDE was associated with the classification and total survival of myeloid leukemia in clinical patients. After transfection of siRNA-CRNDE, the proliferation and cloning ability of U937 cells decreased, while the apoptosis increased (p < 0.01) and cells were arrested in G0-G1 phase. Meanwhile, after transfection of pcDNA-CRNDE, the proliferation ability of U937 cells increased significantly, which indicated that the expression of lncRNA-CRNDE might play an essential role in promoting the proliferation of U937 cells.
LncRNA-CRNDE is highly expressed in the bone marrow tissues of AML patients, and the expression level is negatively correlated with the total survival of those clinical patients. Meanwhile, the expression is higher in FAB type M4 and M5 than that in M1, M2 and M3. LncRNA-CRNDE promotes the proliferation and cell cycle of U937 cells, and inhibits cell apoptosis, which is expected to become a molecular marker for predicting and treating AML.
检测长链非编码 RNA-CRNDE 在急性髓系白血病（AML）患者中的表达及其对急性髓系白血病细胞系 U937 增殖和凋亡的影响。
纳入 81 例初诊 AML 患者，并选择 35 例非恶性血液病患者作为对照。采用实时定量 RT-PCR（qRT-PCR）检测受试者骨髓标本中 lncRNA-CRNDE 的表达，并比较两组间的差异。根据临床资料分析 lncRNA-CRNDE 的表达与临床患者的性别、年龄、分类和总生存期的相关性。培养 U937 细胞和正常人分离的单核细胞，比较急性髓系白血病细胞系 U937 和正常人单核细胞中 lncRNA-CRNDE 的表达。转染 siRNA-CRNDE 和 pcDNA-CRNDE 入 U937 细胞，细胞计数试剂盒-8（CCK-8）检测 U937 细胞增殖，Annexin V/PI 流式细胞术检测细胞凋亡，流式细胞术检测细胞周期。
AML 患者和 U937 细胞中 lncRNA-CRNDE 的表达明显高于非恶性血液病对照组。临床资料结果表明，lncRNA-CRNDE 的表达与临床患者的髓系白血病分类和总生存期有关。转染 siRNA-CRNDE 后，U937 细胞的增殖和克隆能力下降，而凋亡增加（p<0.01），细胞停滞在 G0-G1 期。同时，转染 pcDNA-CRNDE 后，U937 细胞的增殖能力显著增加，表明 lncRNA-CRNDE 的表达可能在促进 U937 细胞增殖中起重要作用。
lncRNA-CRNDE 在 AML 患者的骨髓组织中高表达，表达水平与临床患者的总生存期呈负相关。同时，在 FAB 型 M4 和 M5 中表达高于 M1、M2 和 M3。lncRNA-CRNDE 促进 U937 细胞的增殖和细胞周期，抑制细胞凋亡，有望成为预测和治疗 AML 的分子标志物。
