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[桃棕小叶(棕榈科,桃棕种)品种diamonds - 10的微观形态学]

[Micromorphology of pejibaye leaflets Bactris gasipaes (Arecaceae) var. diamonds-10].

作者信息

Sánchez-Chacón Ethel, Alvarado-Rodríguez Olman, Rodríguez-Arrieta Alexander, Gómez-Alpízar Luis

出版信息

Rev Biol Trop. 2016 Sep;64(3):1273-85.

Abstract

Bactris gasipaes is widely cultivated for the consumption of palm hearts and fruits. The present work describes the micro morphological characteristics of leaflets from adult plants of B. gasipaes, thornless variety Diamantes-10, collected in the Diamantes Experimental Station in Guápiles, Costa Rica. We collected 25 leaflets and analyses were performed with a combination of microscopy techniques: light microscopy, scanning electron microscopy and transmission electron microscopy to study their structure. Our results showed that leaflets have abundant epicuticular wax on adaxial and abaxial surfaces. Analyses from the epidermis indicated that it is composed of isodiametric cells, and it is also evident that hypodermis cells have rectangular shape and are larger than the other epidermal cells. We observed stomata on both surfaces, but they were more abundant in the abaxial surface. On the other hand, the epidermis showed the presence of trichomes with three different morphologies. In the parenchyma, cells are large and not well defined, and we observed the presence of astroesclereids, and compact groups of fiber bundles between parenchyma cells. The central vein has several vascular bundles, arranged in a continuous manner, and they are surrounded by sclerotic tissue; some of these fibers presented live protoplasts. All minor veins showed the same anatomy as the central vein. In these veins, the vessel elements of protoxylem and metaxylem showed scalariform ornaments on their walls. Phloem is located towards the adaxial surface of the vein and we observed sieve and companion cells surrounded by fibers and parenchyma cells. The companion cells presented branched plasmodesmata attached to a sieve element, and in these elements we found protein bodies called P-protein. The main anatomical difference in the leaflets of the var. Diamantes-10, compared to the other varieties of B. gasipaes K, is the lack of thorns; the other morphological features seem to be conserved.

摘要

桃金娘科油棕广泛种植以供食用棕榈心和果实。本研究描述了在哥斯达黎加瓜皮莱斯的钻石实验站收集的无刺品种钻石 - 10成年植株小叶的微观形态特征。我们收集了25片小叶,并结合光学显微镜、扫描电子显微镜和透射电子显微镜等多种显微镜技术进行分析以研究其结构。我们的结果表明,小叶的上表面和下表面都有丰富的表皮蜡质。对表皮的分析表明,它由等径细胞组成,并且也明显可见,皮下细胞呈长方形,比其他表皮细胞大。我们在两个表面都观察到了气孔,但下表面的气孔更为丰富。另一方面,表皮显示存在三种不同形态的毛状体。在薄壁组织中,细胞大且界限不清晰,我们观察到星状石细胞的存在,以及薄壁组织细胞之间紧密排列的纤维束群。中脉有几个维管束,呈连续排列,并被硬化组织包围;其中一些纤维呈现活的原生质体。所有小叶脉的解剖结构与中脉相同。在这些叶脉中,原生木质部和后生木质部的导管分子在其壁上呈现梯纹纹饰。韧皮部位于叶脉的上表面,我们观察到筛管和伴胞被纤维和薄壁组织细胞包围。伴胞呈现出附着在筛管分子上的分支胞间连丝,并且在这些筛管分子中我们发现了称为P蛋白的蛋白质体。与油棕其他品种相比,钻石 - 10品种小叶的主要解剖学差异在于没有刺;其他形态特征似乎得以保留。

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