Gul Saba, Ahmad Mushtaq, Zafar Muhammad, Bahadur Saraj, Sultana Shazia, Ashfaq Shomaila, Ullah Fazal, Kilic Omer, Hassan Fayyaz Ul, Siddiq Zafar
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Technical Sciences Vocational School, Bingol University, Bingol, Turkey.
Microsc Res Tech. 2019 Mar;82(3):206-223. doi: 10.1002/jemt.23157. Epub 2019 Jan 11.
Foliar epidermal features were based on the micromorphology of trichomes types, epidermal cells and stomatal complex. Even though each feature has its own limited taxonomic value but collectively these characteristics may be systematically important especially for the discrimination and identification of complex and problematic taxa. The systematics significance of nonglandular (NGTs) and glandular trichomes (GTs), stomatal complex and epidermal cells of Lamiaceous flora were analyzed by using the light microscopy (LM) and scanning electron microscopy (SEM). Variations on the observed epidermal appendages were divided into two basic types: glandular and nonglandular. GTs can be divided into subtypes: sessile capitate, subsessile capitate, and barrel and sunken. NGTs were also divided into subtypes: dendritic, stellate, conical, falcate, simple and 1-6 cells long having granulate and smooth surface ornamentation. NGTs were the most dominant features of both adaxial and abaxial surfaces of all observed taxa. Vitex negundo, Isodon rugosus, Colebrookea oppositifolia, and Marrubium vulgare could be demarked because of their twisted like appearance of NGTs at the abaxial surface. The Lamiaceae had both hypostomatic and amphistomatic leaf. Stomata were observed as diacytic, anisocytic, and anomocytic. Epidermal cells were found to be irregular, isodiametric, and rectangular. Based on these characters a taxonomic key was developed to delimit the closely related taxa. Distribution and morphology of the foliar epidermal trichomes through SEM highlight an important taxonomic tool used by the taxonomists as an aid to the correct identification of problematic Lamiaceae taxa.
叶表皮特征基于毛状体类型、表皮细胞和气孔复合体的微观形态。尽管每个特征都有其有限的分类学价值，但总体而言，这些特征在系统学上可能具有重要意义，特别是对于复杂和有问题的分类群的鉴别和识别。通过光学显微镜（LM）和扫描电子显微镜（SEM）分析了唇形科植物区系中非腺毛（NGTs）和腺毛（GTs）、气孔复合体和表皮细胞的系统学意义。观察到的表皮附属物的变异分为两种基本类型：腺毛和非腺毛。GTs可分为亚型：无柄头状、近无柄头状、桶状和凹陷状。NGTs也分为亚型：树枝状、星状、圆锥形、镰状、单生以及1 - 6细胞长且表面有颗粒状和平滑纹饰。在所有观察到的分类群中，NGTs是叶正面和背面最主要的特征。黄荆、糙苏、对叶刺蕊草和药用牛至可以因其叶背面NGTs扭曲的外观而被区分开来。唇形科植物的叶既有下气孔型也有双面气孔型。观察到的气孔类型有平列型、不等细胞型和无规则型。表皮细胞呈不规则形、等径形和长方形。基于这些特征编制了一个分类检索表来界定亲缘关系较近的分类群。通过扫描电子显微镜观察叶表皮毛状体的分布和形态，突出了分类学家用于辅助正确识别有问题的唇形科分类群的一种重要分类工具。
