Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Radcliffe Department of Medicine, Medical Sciences Division, University of Oxford, Oxford, United Kingdom.
Antimicrob Agents Chemother. 2018 Apr 26;62(5). doi: 10.1128/AAC.02256-17. Print 2018 May.
Some Kelch mutations of the K13 protein confer increased survival to dihydroartemisinin (DHA)-treated ring-stage parasites. Here, we asked if K13 mutations affect a dormancy phenotype allowing parasites to survive DHA exposure and then sorbitol selection. Although recrudescence from dormancy differed between two distinct parasite lines, it was similar for isogenic lines carrying single-site substitutions in K13. Therefore, K13 mutations do not alter the DHA-sorbitol combined dormancy phenotype; rather, traits from other loci likely determine this phenotype.
一些 Kelch 蛋白的突变使疟原虫对二氢青蒿素（DHA）处理的环状期寄生虫的存活能力增加。在这里，我们想知道 K13 突变是否会影响休眠表型，使寄生虫能够在 DHA 暴露后和山梨醇选择中存活。尽管两种不同的寄生虫系之间的复发从休眠中有所不同，但携带 K13 单点突变的同基因系之间的差异相似。因此，K13 突变不会改变 DHA-山梨醇联合休眠表型；相反，其他基因座的特征可能决定了这种表型。
