基于流式细胞术的恶性疟原虫青蒿素抗性在环状体存活试验中的分析。
Flow cytometry-based analysis of artemisinin-resistant Plasmodium falciparum in the ring-stage survival assay.
作者信息
Amaratunga Chanaki, Neal Aaron T, Fairhurst Rick M
机构信息
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
出版信息
Antimicrob Agents Chemother. 2014 Aug;58(8):4938-40. doi: 10.1128/AAC.02902-14. Epub 2014 May 27.
Abstract
The ring-stage survival assay (RSA) is a powerful tool for phenotyping artemisinin-resistant Plasmodium falciparum but requires experienced microscopists to count viable parasites among 10,000 erythrocytes in Giemsa-stained thin blood smears. Here we describe a rapid flow cytometric assay that accurately counts viable parasites among 250,000 erythrocytes in suspension. This method performs as well as light microscopy and can be used to standardize the collection of RSA data between research groups in laboratory and field settings.
摘要
环状体存活试验（RSA）是用于对青蒿素耐药恶性疟原虫进行表型分析的有力工具，但需要经验丰富的显微镜技术人员在吉姆萨染色的薄血涂片的10,000个红细胞中计数存活的寄生虫。在此，我们描述了一种快速流式细胞术检测方法，该方法可准确计数悬浮液中250,000个红细胞中的存活寄生虫。该方法与光学显微镜的效果相当，可用于在实验室和现场环境中规范各研究组之间RSA数据的收集。
相似文献
1
Flow cytometry-based analysis of artemisinin-resistant Plasmodium falciparum in the ring-stage survival assay.基于流式细胞术的恶性疟原虫青蒿素抗性在环状体存活试验中的分析。
Antimicrob Agents Chemother. 2014 Aug;58(8):4938-40. doi: 10.1128/AAC.02902-14. Epub 2014 May 27.
2
The extended recovery ring-stage survival assay provides a superior association with patient clearance half-life and increases throughput.扩展恢复环阶段存活测定法与患者清除半衰期具有更好的相关性，并提高了通量。
Malar J. 2020 Jan 31;19(1):54. doi: 10.1186/s12936-020-3139-6.
3
Plasmodium falciparum founder populations in western Cambodia have reduced artemisinin sensitivity in vitro.柬埔寨西部的恶性疟原虫奠基人群在体外对青蒿素的敏感性有所降低。
Antimicrob Agents Chemother. 2014 Aug;58(8):4935-7. doi: 10.1128/AAC.03055-14. Epub 2014 May 27.
4
Alternative methods for the Plasmodium falciparum artemisinin ring-stage survival assay with increased simplicity and parasite stage-specificity.用于恶性疟原虫青蒿素环期存活测定的替代方法，具有更高的简易性和寄生虫阶段特异性。
Malar J. 2016 Feb 17;15:94. doi: 10.1186/s12936-016-1148-2.
5
Phenotypic changes in artemisinin-resistant Plasmodium falciparum lines in vitro: evidence for decreased sensitivity to dormancy and growth inhibition.青蒿素耐药恶性疟原虫系的表型变化：对休眠和生长抑制敏感性降低的证据。
Antimicrob Agents Chemother. 2012 Jan;56(1):428-31. doi: 10.1128/AAC.05456-11. Epub 2011 Oct 10.
6
Decreased Artemisinin Sensitivity of across India.印度出现青蒿素敏感性降低现象。
Antimicrob Agents Chemother. 2019 Sep 23;63(10). doi: 10.1128/AAC.00101-19. Print 2019 Oct.
7
Assessment of the induction of dormant ring stages in Plasmodium falciparum parasites by artemisone and artemisone entrapped in Pheroid vesicles in vitro.体外评估青蒿酮及包裹于类脂质体囊泡中的青蒿酮对恶性疟原虫休眠环状体阶段的诱导作用。
Antimicrob Agents Chemother. 2014 Dec;58(12):7579-82. doi: 10.1128/AAC.02707-14. Epub 2014 Oct 6.
8
Plasmodium falciparum in vitro continuous culture conditions: A comparison of parasite susceptibility and tolerance to anti-malarial drugs throughout the asexual intra-erythrocytic life cycle.恶性疟原虫体外连续培养条件：在整个红内无性生殖生命周期中对疟原虫敏感性和抗疟药物耐受性的比较。
Int J Parasitol Drugs Drug Resist. 2017 Dec;7(3):295-302. doi: 10.1016/j.ijpddr.2017.07.001. Epub 2017 Jul 15.
9
Optimal assay design for determining the in vitro sensitivity of ring stage Plasmodium falciparum to artemisinins.用于确定恶性疟原虫环状体期对青蒿素体外敏感性的最佳检测设计。
Int J Parasitol. 2014 Oct 15;44(12):893-9. doi: 10.1016/j.ijpara.2014.07.008. Epub 2014 Aug 23.
10
Role of Plasmodium falciparum Kelch 13 Protein Mutations in P. falciparum Populations from Northeastern Myanmar in Mediating Artemisinin Resistance.恶性疟原虫Kelch13 蛋白突变在缅甸东北部地区恶性疟原虫种群中对青蒿素耐药性的介导作用。
mBio. 2020 Feb 25;11(1):e01134-19. doi: 10.1128/mBio.01134-19.
引用本文的文献
1
Plasmodium falciparum isolates: ex vivo drug response.恶性疟原虫分离株：体外药物反应
J Antimicrob Chemother. 2025 Jul 1;80(7):1813-1822. doi: 10.1093/jac/dkaf129.
2
Malaria parasites undergo a rapid and extensive metamorphosis after invasion of the host erythrocyte.疟原虫侵入宿主红细胞后会经历快速且广泛的形态变化。
EMBO Rep. 2025 Apr 4. doi: 10.1038/s44319-025-00435-3.
3
The extended recovery ring-stage survival assay is a scalable alternative for artemisinin susceptibility phenotyping of fresh isolates.扩展恢复期环形期存活试验是对新鲜分离株进行青蒿素敏感性表型分析的一种可扩展替代方法。
Antimicrob Agents Chemother. 2024 Dec 5;68(12):e0118324. doi: 10.1128/aac.01183-24. Epub 2024 Nov 15.
4
Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites.鉴定疟原虫细胞铁代谢所需的二价金属转运蛋白。
Proc Natl Acad Sci U S A. 2024 Nov 5;121(45):e2411631121. doi: 10.1073/pnas.2411631121. Epub 2024 Oct 28.
5
A kalihinol analog disrupts apicoplast function and vesicular trafficking in malaria.一种卡利辛醇类似物扰乱疟原虫类质体功能和囊泡运输。
Science. 2024 Sep 27;385(6716):eadm7966. doi: 10.1126/science.adm7966.
6
Infection length and host environment influence on Plasmodium falciparum dry season reservoir.感染时长和宿主环境对恶性疟原虫（Plasmodium falciparum）旱季储源的影响。
EMBO Mol Med. 2024 Oct;16(10):2349-2375. doi: 10.1038/s44321-024-00127-w. Epub 2024 Sep 16.
7
Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites.疟原虫细胞铁代谢所需二价金属转运蛋白的鉴定。
bioRxiv. 2024 Jun 10:2024.05.10.587216. doi: 10.1101/2024.05.10.587216.
8
Extended blood stage sensitivity profiles of to doxycycline and tafenoquine, as a model for .以 为例，评估多西环素和他非诺喹的延长红细胞期药敏谱。
Antimicrob Agents Chemother. 2024 May 2;68(5):e0028024. doi: 10.1128/aac.00280-24. Epub 2024 Apr 8.
9
Trio fluorophore-based phenotypic assay for the detection of artemisinin-induced growth-arrested Plasmodium falciparum in human erythrocytes.基于三重荧光团的表型分析方法检测青蒿素诱导的人红细胞内生长受抑的恶性疟原虫。
Sci Rep. 2024 Jan 20;14(1):1802. doi: 10.1038/s41598-024-52414-8.
10
Treatment Failure in a UK Malaria Patient Harboring Genetically Variant Plasmodium falciparum From Uganda With Reduced In Vitro Susceptibility to Artemisinin and Lumefantrine.英国疟疾病例治疗失败，携带源自乌干达具遗传变异的疟原虫 falciparum，对青蒿素和咯萘啶的体外敏感性降低。
Clin Infect Dis. 2024 Feb 17;78(2):445-452. doi: 10.1093/cid/ciad724.
本文引用的文献
1
A molecular marker of artemisinin-resistant Plasmodium falciparum malaria.青蒿素耐药恶性疟原虫的一个分子标记。
Nature. 2014 Jan 2;505(7481):50-5. doi: 10.1038/nature12876. Epub 2013 Dec 18.
2
Novel phenotypic assays for the detection of artemisinin-resistant Plasmodium falciparum malaria in Cambodia: in-vitro and ex-vivo drug-response studies.柬埔寨青蒿素耐药性恶性疟原虫的新型表型检测方法：体外和体内药物反应研究。
Lancet Infect Dis. 2013 Dec;13(12):1043-9. doi: 10.1016/S1473-3099(13)70252-4. Epub 2013 Sep 11.
3
Multiple populations of artemisinin-resistant Plasmodium falciparum in Cambodia.柬埔寨存在多种青蒿素耐药恶性疟原虫种群。
Nat Genet. 2013 Jun;45(6):648-55. doi: 10.1038/ng.2624. Epub 2013 Apr 28.
4
Altered temporal response of malaria parasites determines differential sensitivity to artemisinin.疟原虫时间响应的改变决定了对青蒿素的不同敏感性。
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5157-62. doi: 10.1073/pnas.1217452110. Epub 2013 Feb 19.
5
Artemisinin-resistant Plasmodium falciparum in Pursat province, western Cambodia: a parasite clearance rate study.柬埔寨西部磅士卑省的青蒿素耐药疟原虫：寄生虫清除率研究。
Lancet Infect Dis. 2012 Nov;12(11):851-8. doi: 10.1016/S1473-3099(12)70181-0. Epub 2012 Aug 30.
6
Emergence of artemisinin-resistant malaria on the western border of Thailand: a longitudinal study.泰国西部边境地区青蒿素耐药疟疾的出现：一项纵向研究。
Lancet. 2012 May 26;379(9830):1960-6. doi: 10.1016/S0140-6736(12)60484-X. Epub 2012 Apr 5.
7
Standardizing the measurement of parasite clearance in falciparum malaria: the parasite clearance estimator.标准化恶性疟原虫清除率的测量：寄生虫清除估算器。
Malar J. 2011 Nov 10;10:339. doi: 10.1186/1475-2875-10-339.
8
Identifying apicoplast-targeting antimalarials using high-throughput compatible approaches.利用高通量兼容方法鉴定靶向顶体的抗疟药物。
FASEB J. 2011 Oct;25(10):3583-93. doi: 10.1096/fj.11-187401. Epub 2011 Jul 11.
9
Artemisinin activity against Plasmodium falciparum requires hemoglobin uptake and digestion.青蒿素抗疟原虫活性需要血红蛋白摄取和消化。
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11405-10. doi: 10.1073/pnas.1104063108. Epub 2011 Jun 27.
10
Artemisinin resistance in Plasmodium falciparum malaria.恶性疟原虫疟疾中的青蒿素耐药性。
N Engl J Med. 2009 Jul 30;361(5):455-67. doi: 10.1056/NEJMoa0808859.
Zotero 插件