Drexler H G, Otsuka K, Gaedicke G, Minowada J
Cancer Res. 1986 Dec;46(12 Pt 1):6078-82.
The human leukemia cell lines HL-60, KG-1, KLM-2, ML-3, THP-1, and U-937 were treated with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA). TPA partially or completely inhibited the proliferative activity of the cell cultures. The number of cells with the ability to reduce nitroblue tetrazolium increased in the TPA-treated cell lines HL-60, ML-3, THP-1, and U-937, whereas the cell lines KG-1 and KLM-2 remained nitroblue tetrazolium negative. Except for KG-1 and KLM-2, all TPA-treated cell lines showed varying degrees of strong adherence to plastic surface. The carboxylic esterase, acid phosphatase, hexosaminidase, and lactate dehydrogenase isoenzyme profiles from these cell lines were analyzed by isoelectric focusing on horizontal polyacrylamide gels. The new or stronger expression of an esterase isoenzyme which is specific for monocytes-macrophages was induced in HL-60, ML-3, THP-1, and U-937 but not in KG-1 or KLM-2. The new expression of the tartrate-resistant acid phosphatase isoenzyme was induced in ML-3, THP-1, and U-937. The number of esterase and acid phosphatase isoenzymes and the staining intensity of isoenzymes characteristic for myeloid cells were increased by TPA in all cell lines. The loss of the hexosaminidase I isoenzyme which is a marker of immature hematopoietic cells was noted in KG-1, ML-3, THP-1, and U-937. TPA triggered an increase in number and staining intensity of the lactate dehydrogenase isoenzymes in all cell lines. Some isoenzymatic changes (e.g., monocyte-specific esterase, tartrate-resistant acid phosphatase, hexosaminidase I) appear to correlate with TPA-induced differentiation while other alterations in the isoenzyme patterns do not (e.g., lactate dehydrogenase, other esterase and acid phosphatase isoenzymes). Differentiation of nonmonocytoid cells appears, at the isoenzyme level, to be quite different from that of the monocytoid cell lines.
用人白血病细胞系HL - 60、KG - 1、KLM - 2、ML - 3、THP - 1和U - 937用佛波酯12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）处理。TPA部分或完全抑制细胞培养物的增殖活性。在经TPA处理的细胞系HL - 60、ML - 3、THP - 1和U - 937中，具有还原硝基蓝四氮唑能力的细胞数量增加，而细胞系KG - 1和KLM - 2仍为硝基蓝四氮唑阴性。除KG - 1和KLM - 2外，所有经TPA处理的细胞系对塑料表面均表现出不同程度的强烈黏附。通过在水平聚丙烯酰胺凝胶上进行等电聚焦分析这些细胞系的羧酸酯酶、酸性磷酸酶、己糖胺酶和乳酸脱氢酶同工酶谱。在HL - 60、ML - 3、THP - 1和U - 937中诱导了对单核细胞 - 巨噬细胞特异的酯酶同工酶的新表达或更强表达，但在KG - 1或KLM - 2中未诱导。在ML - 3、THP - 1和U - 937中诱导了抗酒石酸酸性磷酸酶同工酶的新表达。在所有细胞系中，TPA增加了酯酶和酸性磷酸酶同工酶的数量以及髓样细胞特征性同工酶的染色强度。在KG - 1、ML - 3、THP - 1和U - 937中注意到作为未成熟造血细胞标志物的己糖胺酶I同工酶的缺失。TPA引发所有细胞系中乳酸脱氢酶同工酶数量和染色强度的增加。一些同工酶变化（如单核细胞特异性酯酶、抗酒石酸酸性磷酸酶、己糖胺酶I）似乎与TPA诱导的分化相关，而同工酶模式中的其他改变则不然（如乳酸脱氢酶、其他酯酶和酸性磷酸酶同工酶）。在同工酶水平上，非单核细胞系的分化似乎与单核细胞系的分化有很大不同。
