基于具有催化发夹组装的花状载体的细胞内微小RNA的灵敏检测
Sensitive detection of intracellular microRNA based on a flowerlike vector with catalytic hairpin assembly.
作者信息
Liu Jintong, Du Ping, Zhang Jing, Shen Hong, Lei Jianping
机构信息
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
出版信息
Chem Commun (Camb). 2018 Mar 6;54(20):2550-2553. doi: 10.1039/c7cc09579a.
A flowerlike nanovector is developed for the fluorescence imaging and detection of miRNA in live cells with catalytic hairpin assembly. This horned vector provides a high loading efficiency of DNA amplifiers for multiple signal outputs and is applied to detect miRNA-21 in an average single HeLa cell, showing promising application in trace detection of intracellular biomolecules.
一种花状纳米载体被开发用于通过催化发夹组装对活细胞中的miRNA进行荧光成像和检测。这种带角的载体为多种信号输出提供了高负载效率的DNA放大器，并应用于在单个HeLa细胞中检测miRNA-21，在细胞内生物分子的痕量检测中显示出有前景的应用。
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