Chair of Aroma and Smell Research, Emil Fischer Center, Department of Chemistry and Pharmacy, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 9, 91054, Erlangen, Germany.
Department Sensory Analytics, Fraunhofer Institute for Process Engineering and Packaging IVV, Giggenhauser Straße 35, 85354, Freising, Germany.
Anal Bioanal Chem. 2018 Apr;410(10):2585-2595. doi: 10.1007/s00216-018-0929-6. Epub 2018 Feb 20.
Based on the observation that the characteristic odour of inflatable aquatic toys for children is predominantly caused by residues of hazardous organic solvents, the concentrations of cyclohexanone, isophorone and phenol were determined in a selection of 20 products obtained from online suppliers located in Germany. Analytes were extracted with dichloromethane after the addition of non-labelled internal standards, and the volatile fraction was isolated using solvent-assisted flavour evaporation (SAFE). Extracts were then concentrated by Vigreux distillation and analysed by means of gas chromatography with mass spectrometric detection (GC-MS). Furthermore, each sample was evaluated regarding its specific olfactory properties by an expert sensory panel. While some samples did not contain significant amounts of solvents, cyclohexanone concentrations above the lower limit of quantification (LLOQ) were determined in nine samples with six samples containing high concentrations ranging from about 1 to 7 g/kg cyclohexanone. Isophorone concentrations above the LLOQ were observed in eight samples. Thereby, six products contained between 0.3 and 1.6 g/kg isophorone and the remaining two samples contained even about 5 g/kg isophorone, each. Likewise, phenol concentrations exceeded the LLOQ in 14 cases, with four samples containing elevated amounts ranging from about 140 to 280 mg/kg phenol.
基于充气式儿童水上玩具的特征气味主要是由危险有机溶剂残留引起的观察结果，从位于德国的在线供应商处获得的 20 种产品中选择了环己酮、异佛尔酮和苯酚的浓度进行了测定。在添加非标记内标后，用二氯甲烷提取分析物，并用溶剂辅助风味蒸发（SAFE）分离挥发性馏分。然后通过Vigreux 蒸馏浓缩提取物，并通过气相色谱-质谱检测（GC-MS）进行分析。此外，每个样品均由专家感官小组评估其特定的嗅觉特性。虽然一些样品不含有大量的溶剂，但在 9 个样品中检测到环己酮浓度超过定量下限（LLOQ），其中 6 个样品含有浓度高达约 1 至 7 g/kg 的环己酮。在 8 个样品中观察到异佛尔酮浓度超过 LLOQ。因此，有 6 种产品含有 0.3 至 1.6 g/kg 的异佛尔酮，其余 2 种样品甚至含有约 5 g/kg 的异佛尔酮。同样，在 14 种情况下，苯酚浓度超过了 LLOQ，其中 4 种样品含有从约 140 到 280 mg/kg 不等的高浓度苯酚。
